Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/51667
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Potent transcriptional interference by pausing of RNA polymerases over a downstream promoter
Author: Palmer, A.
Ahlgren-Berg, A.
Egan, J.
Dodd, I.
Shearwin, K.
Citation: Molecular Cell, 2009; 34(5):545-555
Publisher: Cell Press
Issue Date: 2009
ISSN: 1097-4164
1097-2765
Statement of
Responsibility: 
Adam C. Palmer, Alexandra Ahlgren-Berg, J. Barry Egan, Ian B. Dodd and Keith E. Shearwin
Abstract: Elongating RNA polymerases (RNAPs) can interfere with transcription from downstream promoters by inhibiting DNA binding by RNAP and activators. However, combining quantitative measurement with mathematical modeling, we show that simple RNAP elongation cannot produce the strong asymmetric interference observed between a natural face-to-face promoter pair in bacteriophage lambda. Pausing of elongating polymerases over the RNAP-binding site of the downstream promoter is demonstrated in vivo and is shown by modeling to account for the increased interference. The model successfully predicts the effects on interference of treatments increasing or reducing pausing. Gene regulation by pausing-enhanced occlusion provides a general and potentially widespread mechanism by which even weak converging or tandem transcription, either coding or noncoding, can bring about strong in cis repression.
Keywords: Escherichia coli; Bacteriophage lambda; DNA-Directed RNA Polymerases; Transcription, Genetic; Gene Expression Regulation; Binding Sites; Mutation; Models, Genetic; Promoter Regions, Genetic
Rights: © 2009 Elsevier Inc.
RMID: 0020090925
DOI: 10.1016/j.molcel.2009.04.018
Description (link): http://www.cell.com/molecular-cell/home
Appears in Collections:Molecular and Biomedical Science publications

Files in This Item:
File Description SizeFormat 
hdl_51667.pdfAccepted version1.01 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.