Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/51667
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Potent transcriptional interference by pausing of RNA polymerases over a downstream promoter |
Author: | Palmer, A. Ahlgren-Berg, A. Egan, J. Dodd, I. Shearwin, K. |
Citation: | Molecular Cell, 2009; 34(5):545-555 |
Publisher: | Cell Press |
Issue Date: | 2009 |
ISSN: | 1097-4164 1097-2765 |
Statement of Responsibility: | Adam C. Palmer, Alexandra Ahlgren-Berg, J. Barry Egan, Ian B. Dodd and Keith E. Shearwin |
Abstract: | Elongating RNA polymerases (RNAPs) can interfere with transcription from downstream promoters by inhibiting DNA binding by RNAP and activators. However, combining quantitative measurement with mathematical modeling, we show that simple RNAP elongation cannot produce the strong asymmetric interference observed between a natural face-to-face promoter pair in bacteriophage lambda. Pausing of elongating polymerases over the RNAP-binding site of the downstream promoter is demonstrated in vivo and is shown by modeling to account for the increased interference. The model successfully predicts the effects on interference of treatments increasing or reducing pausing. Gene regulation by pausing-enhanced occlusion provides a general and potentially widespread mechanism by which even weak converging or tandem transcription, either coding or noncoding, can bring about strong in cis repression. |
Keywords: | Escherichia coli Bacteriophage lambda DNA-Directed RNA Polymerases Transcription, Genetic Gene Expression Regulation Binding Sites Mutation Models, Genetic Promoter Regions, Genetic |
Rights: | © 2009 Elsevier Inc. |
DOI: | 10.1016/j.molcel.2009.04.018 |
Grant ID: | ARC |
Description (link): | http://www.cell.com/molecular-cell/home |
Published version: | http://dx.doi.org/10.1016/j.molcel.2009.04.018 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_51667.pdf | Accepted version | 1.01 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.