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|Title:||The impact of hydration changes in fresh bio-tissue on THz spectroscopic measurements|
|Citation:||Physics in Medicine and Biology, 2008; 53(13):3501-3517|
|Publisher:||IOP Publishing Ltd|
|G. M. Png, J. W. Choi, B. W.-H. Ng, S. P. Mickan, D. Abbott and X.-C. Zhang|
|Abstract:||We present a study of how residual hydration in fresh rat tissue samples can vastly alter their extracted terahertz (THz) optical properties and influence their health assessment. Fresh (as opposed to preserved) tissue most closely mimics in vivo conditions, but high water content creates many challenges for tissue handling and THz measurement. Our THz measurements of fresh tissue over time highlight the effect of tissue hydration on tissue texture and dimension, the latter directly influencing the accuracy of calculated optical properties. We then introduce lyophilization (freeze drying) as a viable solution for overcoming hydration and freshness problems. Lyophilization removes large amounts of water while retaining sample freshness. In addition, lyophilized tissue samples are easy to handle and their textures and dimensions do not vary over time, allowing for consistent and stable THz measurements. A comparison of lyophilized and fresh tissue shows for the first time that freeze drying may be one way of overcoming tissue hydration issues while preserving tissue cellular structure. Finally, we compare THz measurements from fresh tissue against necrotic tissue to verify freshness over time. Indeed, THz measurements from fresh and necrotic tissues show marked differences.|
|Keywords:||Animals; Rats; Water; Necrosis; Spectrum Analysis; Freeze Drying; Tissue Preservation; Tissue Survival; Models, Theoretical; Optics and Photonics; Microwaves; Time Factors|
|Appears in Collections:||Electrical and Electronic Engineering publications|
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