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|Title:||Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex|
|Citation:||Acta Crystallographica. Section F: Structural Biology and Crystallization Communications Online, 2008; 64(Part 8):711-714|
|G. Hansen, T. R. Hercus, Y. Xu, A. F. Lopez, M. W. Parker and W. J. McKinstry|
|Abstract:||Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific alpha (GMRalpha) and common beta (betac) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRalpha and betac are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRalpha subunit and either betac or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the betac subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6(3)22 and diffracted to 3.3 A resolution.|
|Keywords:||arthritis; asthma; cytokines; cytokine receptor complex; GM-CSF; interleukins; leukaemia; signalling.|
|Appears in Collections:||Medicine publications|
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