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Type: Journal article
Title: Water movement into dormant and non-dormant wheat (Triticum aestivum L.) grains
Author: Rathjen, J.
Strounina, E.
Mares, D.
Citation: Journal of Experimental Botany, 2009; 60(6):1619-1631
Publisher: Oxford Univ Press
Issue Date: 2009
ISSN: 0022-0957
Statement of
Judith R. Rathjen, Ekaterina V. Strounina and Daryl J. Mares
Abstract: The movement of water into harvest-ripe grains of dormant and non-dormant genotypes of wheat (Triticum aestivum L.) was investigated using Magnetic Resonance Micro-Imaging (MRMI). Images of virtual sections, both longitudinal and transverse, throughout the grain were collected at intervals after the start of imbibition and used to reconstruct a picture of water location within the different grain tissues and changes over time. The observations were supplemented by the weighing measurements of water content and imbibition of grains in water containing I(2)/KI which stains starch and lipid, thereby acting as a marker for water. In closely related genotypes, with either a dormant or a non-dormant phenotype, neither the rate of increase in water content nor the pattern of water distribution within the grain was significantly different until 18 h, when germination became apparent in the non-dormant genotype. Water entered the embryo and scutellum during the very early stages of imbibition through the micropyle and by 2 h water was clearly evident in the micropyle channel. After 12 h of imbibition, embryo structures such as the coleoptile and radicle were clearly distinguished. Although water accumulated between the inner (seed coat) and outer (pericarp) layers of the coat surrounding the grain, there was no evidence for movement of water directly across the coat and into the underlying starchy endosperm.
Keywords: Dormancy; germination; imbibition; MRI; Triticum aestivum; water; wheat
Rights: © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020090629
DOI: 10.1093/jxb/erp037
Appears in Collections:Agriculture, Food and Wine publications

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