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https://hdl.handle.net/2440/53643
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Type: | Journal article |
Title: | A specific anti-aire antibody reveals aire expression is restricted to medullary thymic epithelial cells and not expressed in periphery |
Author: | Hubert, F. Kinkel, S. Webster, K. Cannon, P. Crewther, P. Proeitto, A. Wu, L. Heath, W. Scott, H. |
Citation: | Journal of Immunology, 2008; 180(6):3824-3832 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2008 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Francois-Xavier Hubert, Sarah A. Kinkel, Kylie E. Webster, Ping Cannon, Pauline E. Crewther, Anna I. Proeitto, Li Wu, William R. Heath, and Hamish S. Scott |
Abstract: | Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy is an autoimmune disorder caused by mutations in the autoimmune regulator gene AIRE. We examined the expression of Aire in different organs (thymus, spleen, and lymph nodes) in C57BL/6 mice, using a novel rat mAb, specific for murine Aire. Using flow cytometry, directly fluorochrome-labeled mAb revealed Aire expression in a rare thymic cellular subset that was CD45–, expressed low levels of Ly51, and was high for MHC-II and EpCam. This subset also expressed a specific pattern of costimulatory molecules, including CD40, CD80, and PD-L1. Immunohistochemical analysis revealed that Aire+ cells were specifically localized to the thymus or, more precisely, to the cortico-medulla junction and medulla, correlating with the site of negative selection. Although in agreement with previous studies, low levels of Aire mRNA was detected in all dendritic cell subtypes however lacZ staining, immunohistochemistry and flow cytometry failed to detect Aire protein. At a cellular level, Aire was expressed in perinuclear speckles within the nucleus. This report provides the first detailed analysis of Aire protein expression, highlighting the precise location at both the tissue and cellular level. |
Keywords: | Thymus Gland Cell Nucleus Epithelial Cells Animals Mice, Inbred C57BL Mice, Transgenic Mice, Knockout Mice Rats Transcription Factors Antibodies, Monoclonal Immunophenotyping Quantum Dots Antibody Specificity Antigen-Antibody Reactions Amino Acid Sequence Molecular Sequence Data |
Description: | Copyright © 2008 by The American Association of Immunologists, Inc. All rights reserved. |
DOI: | 10.4049/jimmunol.180.6.3824 |
Published version: | http://www.jimmunol.org/cgi/content/abstract/180/6/3824 |
Appears in Collections: | Aurora harvest Medicine publications |
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