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Type: Journal article
Title: A specific anti-aire antibody reveals aire expression is restricted to medullary thymic epithelial cells and not expressed in periphery
Author: Hubert, F.
Kinkel, S.
Webster, K.
Cannon, P.
Crewther, P.
Proeitto, A.
Wu, L.
Heath, W.
Scott, H.
Citation: Journal of Immunology, 2008; 180(6):3824-3832
Publisher: Amer Assoc Immunologists
Issue Date: 2008
ISSN: 0022-1767
Statement of
Francois-Xavier Hubert, Sarah A. Kinkel, Kylie E. Webster, Ping Cannon, Pauline E. Crewther, Anna I. Proeitto, Li Wu, William R. Heath, and Hamish S. Scott
Abstract: Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy is an autoimmune disorder caused by mutations in the autoimmune regulator gene AIRE. We examined the expression of Aire in different organs (thymus, spleen, and lymph nodes) in C57BL/6 mice, using a novel rat mAb, specific for murine Aire. Using flow cytometry, directly fluorochrome-labeled mAb revealed Aire expression in a rare thymic cellular subset that was CD45–, expressed low levels of Ly51, and was high for MHC-II and EpCam. This subset also expressed a specific pattern of costimulatory molecules, including CD40, CD80, and PD-L1. Immunohistochemical analysis revealed that Aire+ cells were specifically localized to the thymus or, more precisely, to the cortico-medulla junction and medulla, correlating with the site of negative selection. Although in agreement with previous studies, low levels of Aire mRNA was detected in all dendritic cell subtypes however lacZ staining, immunohistochemistry and flow cytometry failed to detect Aire protein. At a cellular level, Aire was expressed in perinuclear speckles within the nucleus. This report provides the first detailed analysis of Aire protein expression, highlighting the precise location at both the tissue and cellular level.
Keywords: Thymus Gland
Cell Nucleus
Epithelial Cells
Mice, Inbred C57BL
Mice, Transgenic
Mice, Knockout
Transcription Factors
Antibodies, Monoclonal
Quantum Dots
Antibody Specificity
Antigen-Antibody Reactions
Amino Acid Sequence
Molecular Sequence Data
Description: Copyright © 2008 by The American Association of Immunologists, Inc. All rights reserved.
DOI: 10.4049/jimmunol.180.6.3824
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