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|Title:||The in situ physiology of Skermania piniformis in foams in Australian activated sludge plants|
|Citation:||Environmental Microbiology, 2006; 8(10):1712-1720|
|Publisher:||Blackwell Science Ltd|
|Kathryn L. Eales, Jeppe L. Nielsen, Elizabeth M. Seviour, Per H. Nielsen and Robert J. Seviour|
|Abstract:||<jats:title>Summary</jats:title><jats:p>The <jats:italic>in situ</jats:italic> physiology of the filamentous bacterium <jats:italic>Skermania piniformis</jats:italic> frequently seen in activated sludge foams in Australia was investigated. An oligonucleotide probe, Spin1449, targeting the 16S rRNA of <jats:italic>S. piniformis</jats:italic> was designed for its identification by fluorescence <jats:italic>in situ</jats:italic> hybridization (FISH), validated with pure cultures and applied successfully to foam samples from two geographically distant Australian plants. While filaments of this bacterium appeared to be comparatively hydrophobic, the organism had no clear preference for hydrophobic or hydrophilic substrates. In both foams examined using microautoradiography (MAR), filaments selectively took up substrates under aerobic and anoxic (NO<jats:sub>3</jats:sub><jats:sup>–</jats:sup>) but not anaerobic or anoxic (NO<jats:sub>2</jats:sub><jats:sup>–</jats:sup>) conditions. <jats:italic>Skermania piniformis</jats:italic> assimilated oleic acid, palmitic acid, glycerol and glycine. Ectoenzyme activities detected suggest that <jats:italic>S. piniformis</jats:italic> has an ability to assimilate a greater range of substrates than might be concluded from the MAR data obtained here. Based on the substrate uptake data presented here, an anaerobic selector may work for controlling <jats:italic>S. piniformis</jats:italic> in activated sludge systems.</jats:p>|
|Appears in Collections:||Agriculture, Food and Wine publications|
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