Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/55731
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Type: Journal article
Title: G-protein-coupled receptors in drug discovery: nanosizing using cell-free technologies and molecular biology approaches
Author: Leifert, W.
Aloia, A.
Bucco, O.
Glatz, R.
McMurchie, E.
Citation: Journal of Biomolecular Screening, 2005; 10(8):765-779
Publisher: Mary Ann Liebert Inc Publ
Issue Date: 2005
ISSN: 1087-0571
1552-454X
Statement of
Responsibility: 
Wayne R. Leifert, Amanda L. Aloia, Olgatina Bucco, Richard V. Glatz, and Edward J. Mcmurchie
Abstract: Signal transduction by G-protein-coupled receptors (GPCRs) underpins a multitude of physiological processes. Ligand recognition by the receptor leads to activation of a genericmolecular switch involving heterotrimeric G-proteins and guanine nucleotides. Signal transduction has been studied extensively with both cell-based systems and assays comprising isolated signaling components. Interest and commercial investment in GPCRs in areas such as drug targets, orphan receptors, highthroughput screening, biosensors, and so on will focus greater attention on assay development to allow for miniaturization, ultra-high throughput and, eventually, microarray/biochip assay formats. Although cell-based assays are adequate for many GPCRs, it is likely that these formatswill limit the development of higher density GPCRassay platforms mandatory for other applications. Stable, robust, cell-free signaling assemblies comprising receptor and appropriate molecular switching components will form the basis of future GPCR assay platforms adaptable for such applications as microarrays. The authors review current cell-free GPCR assay technologies and molecular biological approaches for construction of novel, functional GPCR assays.
Keywords: GPCR; G-proteins; HTS; cell-free; nanotechnology
Description: © 2005 The Society for Biomolecular Screening
RMID: 0020092100
DOI: 10.1177/1087057105280517
Appears in Collections:Agriculture, Food and Wine publications

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