Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/55803
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Type: Journal article
Title: PerR controls Mn-dependent resistance to oxidative stress in Neisseria gonorrhoeae
Author: Wu, H.J.
Seib, K.
Srikhanta, Y.
Kidd, S.
Edwards, J.
Maguire, T.
Grimmond, S.
Apicella, M.
McEwan, A.
Jennings, M.
Citation: Molecular Microbiology, 2006; 60(2):401-416
Publisher: Blackwell Publishing Ltd
Issue Date: 2006
ISSN: 0950-382X
1365-2958
Statement of
Responsibility: 
Hsing-Ju Wu, Kate L. Seib, Yogitha N. Srikhanta, Stephen P. Kidd, Jennifer L. Edwards, Tina L. Maguire, Sean M. Grimmond, Michael A. Apicella, Alastair G. McEwan and Michael P. Jennings
Abstract: In previous studies it has been established that resistance to superoxide by Neisseria gonorrhoeae is dependent on the accumulation of Mn(II) ions involving the ABC transporter, MntABC. A mutant strain lacking the periplasmic binding protein component (MntC) of this transport system is hypersensitive to killing by superoxide anion. In this study the mntC mutant was found to be more sensitive to H2O2 killing than the wild-type. Analysis of regulation of MntC expression revealed that it was de-repressed under low Mn(II) conditions. The N. gonorrhoeae mntABC locus lacks the mntR repressor typically found associated with this locus in other organisms. A search for a candidate regulator of mntABC expression revealed a homologue of PerR, a Mn-dependent peroxide-responsive regulator found in Gram-positive organisms. A perR mutant expressed more MntC protein than wild-type, and expression was independent of Mn(II), consistent with a role for PerR as a repressor of mntABC expression. The PerR regulon of N. gonorrhoeae was defined by microarray analysis and includes ribosomal proteins, TonB-dependent receptors and an alcohol dehydrogenase. Both the mntC and perR mutants had reduced intracellular survival in a human cervical epithelial cell model.
RMID: 0020092899
DOI: 10.1111/j.1365-2958.2006.05079.x
Appears in Collections:Molecular and Biomedical Science publications

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