Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/55844
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Glutathione-dependent alcohol dehydrogenase AdhC is required for defense against nitrosative stress in Haemophilus influenzae. |
Author: | Kidd, S. Jiang, D. Jennings, M. McEwan, A. |
Citation: | Infection and Immunity, 2007; 75(9):4506-4513 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2007 |
ISSN: | 0019-9567 1098-5522 |
Statement of Responsibility: | Kidd, SP; Jiang, D; Jennings, MP. and McEwan, AG. |
Abstract: | In Haemophilus influenzae Rd KW20, we identified a gene, adhC, which encodes a class III alcohol dehydrogenase (AdhC) and has S-nitrosoglutathione reductase activity. adhC exists on an operon with estD, which encodes an esterase. Divergent to the adhC-estD operon is the Haemophilus influenzae nmlR gene (nmlR(HI)), which encodes a MerR family regulator that is homologous to the Neisseria MerR-like regulator (NmlR). Analysis of an nmlR(HI) mutant indicated that expression of the adhC-estD operon is regulated by NmlR(HI) in strain Rd KW20. Chromosomal inactivation of either adhC or nmlR(HI) resulted in sensitivity to S-nitrosoglutathione and decreased S-nitrosoglutathione reductase activity. Examination of the NmlR(HI)-AdhC system in the genome sequences of nontypeable H. influenzae strains R2846, R2866, and 86-028NP identified significant variations. The adhC gene of 86-028NP was predicted to be nonfunctional due to a premature stop codon. Polymorphisms in the operator/promoter region of R2866 resulted in reduced enzyme activity. This correlated with an increased sensitivity to S-nitrosoglutathione. The adhC-nmlR(HI) system was examined in thirty-three clinical isolates (both capsular and nontypeable strains). Nucleic acid sequence data showed that only strain 86-028NP contained a premature stop codon. There were some variations in the DNA sequence of the operator/promoter region which altered the nmlR(HI) promoter. However, the clinical isolates still possessed S-nitrosoglutathione reductase activity and showed at least the equivalent ability to grow in the presence of S-nitrosoglutathione as Rd KW20. These data suggest that the nmlR(HI)-adhC system has a role in the defense against nitrosative stress in Haemophilus influenzae. |
Keywords: | Haemophilus influenzae Reactive Nitrogen Species S-Nitrosoglutathione Aldehyde Oxidoreductases Transcription Factors Gene Expression Regulation, Bacterial Base Sequence Oxidative Stress Molecular Sequence Data |
DOI: | 10.1128/IAI.00487-07 |
Description (link): | http://www.ncbi.nlm.nih.gov/sites/entrez |
Published version: | http://dx.doi.org/10.1128/iai.00487-07 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.