Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/56695
Type: | Journal article |
Title: | DNA from Buccal Swabs Suitable for High-Throughput SNP Multiplex Analysis |
Author: | McMichael, G. Gibson, C. O'Callaghan, M. Goldwater, P. Dekker, G. Haan, E. MacLennan, A. |
Citation: | Journal of Biomolecular Techniques, 2009; 20(5):232-235 |
Publisher: | The Association of Biomolecular Resource Facilities |
Issue Date: | 2009 |
ISSN: | 1524-0215 1943-4731 |
Statement of Responsibility: | Gai L. McMichael, Catherine S. Gibson, Michael E. O’Callaghan, Paul N. Goldwater, Gustaaf A. Dekker, Eric A. Haan, Alastair H. MacLennan, and for the South Australian Cerebral Palsy Research Group |
Abstract: | We sought a convenient and reliable method for collection of genetic material that is inexpensive and noninvasive and suitable for self-collection and mailing and a compatible, commercial DNA extraction protocol to meet quantitative and qualitative requirements for high-throughput single nucleotide polymorphism (SNP) multiplex analysis on an automated platform. Buccal swabs were collected from 34 individuals as part of a pilot study to test commercially available buccal swabs and DNA extraction kits. DNA was quantified on a spectrofluorometer with Picogreen dsDNA prior to testing the DNA integrity with predesigned SNP multiplex assays. Based on the pilot study results, the Catch-All swabs and Isohelix buccal DNA isolation kit were selected for our high-throughput application and extended to a further 1140 samples as part of a large cohort study. The average DNA yield in the pilot study (n=34) was 1.94 μg ± 0.54 with a 94% genotyping pass rate. For the high-throughput application (n=1140), the average DNA yield was 2.44 μg ± 1.74 with a ≥93% genotyping pass rate. The Catch-All buccal swabs are a convenient and cost-effective alternative to blood sampling. Combined with the Isohelix buccal DNA isolation kit, they provided DNA of sufficient quantity and quality for high-throughput SNP multiplex analysis. |
Keywords: | single nucleotide polymorphism |
Description: | Copyright © 2009 Association of Biomolecular Resource Facilities |
Published version: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777348/?report=abstract |
Appears in Collections: | Aurora harvest 5 Cerebral Palsy Research Group publications Obstetrics and Gynaecology publications |
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