Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/61228
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Enrichment for STRO-1 expression enhances the cardiovascular paracrine activity of human bone marrow-derived mesenchymal cell populations |
Author: | Psaltis, P. Paton, S. See, F. Arthur, A. Martin, S. Itescu, S. Worthley, S. Gronthos, S. Zannettino, A. |
Citation: | Journal of Cellular Physiology, 2010; 223(2):530-540 |
Publisher: | Wiley-Liss |
Issue Date: | 2010 |
ISSN: | 0021-9541 1097-4652 |
Statement of Responsibility: | P.J. Psaltis, S. Paton, F. See, A. Arthur, S. Martin, S. Itescu, S.G. Worthley, S. Gronthos and A.C.W. Zannettino |
Abstract: | The cardiovascular therapeutic potential of bone marrow mesenchymal stromal/stem cells (MSC) is largely mediated by paracrine effects. Traditional preparation of MSC has involved plastic adherence-isolation. In contrast, prospective immunoselection aims to improve cell isolation by enriching for mesenchymal precursor cells (MPC) at higher purity. This study compared the biological characteristics and cardiovascular trophic activity of plastic adherence-isolated MSC (PA-MSC) and MPC prepared from the same human donors by immunoselection for stromal precursor antigen-1 (STRO-1). Compared to PA-MSC, STRO-1-MPC displayed greater (1) clonogenicity, (2) proliferative capacity, (3) multilineage differentiation potential, and (4) mRNA expression of mesenchymal stem cell-related transcripts. In vitro assays demonstrated that conditioned medium from STRO-1-MPC had greater paracrine activity than PA-MSC, with respect to cardiac cell proliferation and migration and endothelial cell migration and tube formation. In keeping with this, STRO-1-MPC exhibited higher gene and protein expression of CXCL12 and HGF. Inhibition of these cytokines attenuated endothelial tube formation and cardiac cell proliferation, respectively. Paracrine responses were enhanced by using supernatant from STRO-1(Bright) MPC and diminished with STRO-1(Dim) conditioned medium. Together, these findings indicate that prospective isolation gives rise to mesenchymal progeny that maintain a higher proportion of immature precursor cells compared to traditional plastic adherence-isolation. Enrichment for STRO-1 is also accompanied by increased expression of cardiovascular-relevant cytokines and enhanced trophic activity. Immunoselection thus provides a strategy for improving the cardiovascular reparative potential of mesenchymal cells. |
Keywords: | Bone Marrow Cells Cells, Cultured Endothelial Cells Myocytes, Cardiac Mesenchymal Stem Cells Multipotent Stem Cells Humans Cardiovascular Diseases Plastics RNA, Messenger Antigens, Surface Culture Media, Conditioned Mesenchymal Stem Cell Transplantation Cell Culture Techniques Immunomagnetic Separation Colony-Forming Units Assay Regeneration Cell Adhesion Paracrine Communication Cell Differentiation Cell Proliferation Cell Movement Biomarkers |
Rights: | Copyright © 2010 Wiley-Liss, Inc. |
DOI: | 10.1002/jcp.22081 |
Published version: | http://dx.doi.org/10.1002/jcp.22081 |
Appears in Collections: | Aurora harvest 5 Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.