Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/61879
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dc.contributor.authorRicciardelli, C.en
dc.contributor.authorBianco-Miotto, T.en
dc.contributor.authorJindal, S.en
dc.contributor.authorDodd, T.en
dc.contributor.authorCohen, P.en
dc.contributor.authorMarshall, V.en
dc.contributor.authorSutherland, P.en
dc.contributor.authorSamaratunga, H.en
dc.contributor.authorKench, J.en
dc.contributor.authorDong, Y.en
dc.contributor.authorWang, H.en
dc.contributor.authorClements, J.en
dc.contributor.authorRisbridger, G.en
dc.contributor.authorSutherland, R.en
dc.contributor.authorTilley, W.en
dc.contributor.authorHorsfall, D.en
dc.date.issued2010en
dc.identifier.citationCancer Epidemiology Biomarkers & Prevention, 2010; 19(7):1755-1765en
dc.identifier.issn1055-9965en
dc.identifier.issn1538-7755en
dc.identifier.urihttp://hdl.handle.net/2440/61879-
dc.description.abstractBACKGROUND: Knowledge of preanalytic conditions that biospecimens are subjected to is critically important because novel surgical procedures, tissue sampling, handling, and storage might affect biomarker expression or invalidate tissue samples as analytes for some technologies. METHODS: We investigated differences in RNA quality, gene expression by quantitative real-time PCR, and immunoreactive protein expression of selected prostate cancer biomarkers between tissues from retropubic radical prostatectomy (RRP) and robot-assisted laparoscopic prostatectomy (RALP). Sections of tissue microarray of 23 RALP and 22 RRP samples were stained with antibodies to androgen receptor (AR) and prostate-specific antigen (PSA) as intersite controls, and 14 other candidate biomarkers of research interest to three laboratories within the Australian Prostate Cancer BioResource tissue banking network. Quantitative real-time PCR was done for AR, PSA (KLK3), KLK2, KLK4, and HIF1A on RNA extracted from five RALP and five RRP frozen tissue cores. RESULTS: No histologic differences were observed between RALP and RRP tissue. Biomarker staining grouped these samples into those with increased (PSA, CK8/18, CKHMW, KLK4), decreased (KLK2, KLK14), or no change in expression (AR, ghrelin, Ki67, PCNA, VEGF-C, PAR2, YB1, p63, versican, and chondroitin 0-sulfate) in RALP compared with RRP tissue. No difference in RNA quality or gene expression was detected between RALP and RRP tissue. CONCLUSIONS: Changes in biomarker expression between RALP and RRP tissue exist at the immunoreactive protein level, but the etiology is unclear. IMPACT: Future studies should account for changes in biomarker expression when using RALP tissues, and mixed cohorts of RALP and RRP tissue should be avoided.en
dc.description.statementofresponsibilityCarmela Ricciardelli, Tina Bianco-Miotto, Shalini Jindal, Thomas J. Dodd, Penelope A. Cohen, Villis R. Marshall, Peter D. Sutherland, Hemamali Samaratunga, James G. Kench, Ying Dong, Hong Wang, Judith A. Clements, Gail P. Risbridger, Robert L. Sutherland, Wayne D. Tilley and David J. Horsfall, for the Australian Prostate Cancer BioResourceen
dc.language.isoenen
dc.publisherAmer Assoc Cancer Researchen
dc.rights© 2010 American Association for Cancer Research.en
dc.subjectAustralian Prostate Cancer BioResource; Humans; Prostatic Neoplasms; Prostate-Specific Antigen; Receptors, Androgen; RNA, Neoplasm; Tumor Markers, Biological; Laparoscopy; Specimen Handling; Prostatectomy; Immunohistochemistry; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression; Robotics; Aged; Middle Aged; Maleen
dc.titleComparative biomarker expression and RNA integrity in biospecimens derived from radical retropubic and robot-assisted laparoscopic prostatectomiesen
dc.typeJournal articleen
dc.identifier.rmid0020100285en
dc.identifier.doi10.1158/1055-9965.EPI-10-0059en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/290546en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/443234en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/349457en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/535903en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/390125en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/384104en
dc.identifier.pubid33665-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidRicciardelli, C. [0000-0001-7415-1854]en
dc.identifier.orcidBianco-Miotto, T. [0000-0002-8431-5338]en
dc.identifier.orcidTilley, W. [0000-0003-1893-2626]en
Appears in Collections:Obstetrics and Gynaecology publications

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