Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Feasibility of high-density electrophysiological study using multiple-electrode array in isolated small animal atria|
|Citation:||Clinical and Experimental Pharmacology and Physiology, 2010; 37(10):1023-1027|
|Publisher:||Blackwell Publishing Asia|
|Dennis H. Lau, Lorraine Mackenzie, Nicholas J. Shipp, Pawel Kuklik, Hany Dimitri, Bruce L.W. Lobb, Muayad Alasady, Han S. Lim, Douglas R. Kelly, Anthony G. Brooks, David A. Saint and Prashanthan Sanders|
|Abstract:||1. High-density cardiac electrophysiological study (EPS) of small animal atria has been limited to optical mapping techniques, which require complex and expensive equipment setup. We aim to evaluate the feasibility of carrying out EPS in isolated atrial tissues using a custom made high-density multiple-electrode array (MEA). 2. Isolated rat atrial preparations were studied. The MEA (4 × 5 mm) consisted of 90 silver chloride coated electrodes (0.1 mm diameter, 0.5 mm pitch) and was connected to a conventional EP system yielding 80 bipolar signals. Atrial tissues were placed over the MEA in a dish bubbled with 100% oxygen and superfused with modified HEPES solution at pH 7.35 and 37°C. Then, 1 mmol of 2,3-butanedione monoxime was added to suppress motion artifacts from muscle contractions. Custom plaque analysis software was used for offline conduction analysis. 3. Isolated atrial tissues showed good viability of > 30 min, allowing ample time for complete EPS. High quality electrograms with excellent signal to noise ratio were obtained. All electrophysiological parameters showed good reproducibility: effective refractory period, conduction velocity and heterogeneity index. Tachycardia was also inducible in these normal atria. 4. The present study shows the feasibility of performing high-density EPS of small isolated atrial tissues with a conventional electrode-based technique. The MEA system is compatible with standard electrophysiology recording systems and provides a novel, inexpensive option for detailed EPS in small animal models. In particular, it presents new research avenues to further explore the mechanisms of atrial arrhythmias in various transgenic and knockout rodent models.|
|Keywords:||atria; high-density mapping; multiple-electrode array|
|Rights:||© 2010 The Authors. Clinical and Experimental Pharmacology and Physiology © 2010 Blackwell Publishing Asia Pty Ltd.|
|Appears in Collections:||Medicine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.