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https://hdl.handle.net/2440/62284
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Type: | Journal article |
Title: | Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development |
Author: | Dunning, K. Cashman, K. Russell, D. Thompson, J. Norman, R. Robker, R. |
Citation: | Biology of Reproduction, 2010; 83(6):909-918 |
Publisher: | Soc Study Reproduction |
Issue Date: | 2010 |
ISSN: | 0006-3363 1529-7268 |
Statement of Responsibility: | Kylie R. Dunning, Kara Cashman, Darryl L. Russell, Jeremy G. Thompson, Robert J. Norman, and Rebecca L. Robker |
Abstract: | Oocyte and embryo metabolism are closely linked with their subsequent developmental capacity. Lipids are a potent source of cellular energy, yet little is known about lipid metabolism during oocyte maturation and early embryo development. Generation of ATP from lipids occurs within mitochondria via beta-oxidation of fatty acids, with the rate-limiting step catalyzed by carnitine palmitoyl transferase I (CPT1B), a process also requiring carnitine. We sought to investigate the regulation and role of beta-oxidation during oocyte maturation and preimplantation development. Expression of Cpt1b mRNA, assessed by real-time RT-PCR in murine cumulus-oocyte complexes (COCs), increased following hormonal induction of oocyte maturation and ovulation in vivo with human chorionic gonadotropin (5 IU) and in embryos reaching the blastocyst stage. Beta-oxidation, measured by the production of 3H2O from [3H]palmitic acid, was significantly increased over that in immature COCs following induction of maturation in vitro with epidermal growth factor (3 ng/ml) and follicle-stimulating hormone (50 mIU/ml). The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or l-carnitine, respectively. Inhibition of beta-oxidation during oocyte maturation or zygote cleavage impaired subsequent blastocyst development. In contrast, l-carnitine supplementation during oocyte maturation significantly increased beta-oxidation, improved developmental competence, and in the absence of a carbohydrate energy supply, significantly increased 2-cell cleavage. Thus, carnitine is an important cofactor for developing oocytes, and fatty acids are an important energy source for oocyte and embryo development. |
Keywords: | beta-oxidation CPT1B embryo development fatty acid oxidation oocyte maturation |
Rights: | © 2010 by the Society for the Study of Reproduction, Inc. |
DOI: | 10.1095/biolreprod.110.084145 |
Published version: | http://dx.doi.org/10.1095/biolreprod.110.084145 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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