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https://hdl.handle.net/2440/63227
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dc.contributor.author | Wijesundara, D. | - |
dc.contributor.author | Kumar, S. | - |
dc.contributor.author | Alsharifi, M. | - |
dc.contributor.author | Mullbacher, A. | - |
dc.contributor.author | Regner, M. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | International Immunology, 2010; 22(9):757-767 | - |
dc.identifier.issn | 0953-8178 | - |
dc.identifier.issn | 1460-2377 | - |
dc.identifier.uri | http://hdl.handle.net/2440/63227 | - |
dc.description.abstract | Type-I IFN (IFN-I) are highly pleiotropic cytokines known to modulate immune responses and play an early central role in mediating antiviral defenses. We have shown that IFN-I mediate transient up-regulation of a distinct subset of lymphocyte surface activation markers on both B and T cells in vivo independent of cognate antigen: a state referred to as ‘partial lymphocyte activation’. Here we investigated in vitro the possibility that partial lymphocyte activation may serve to lower the antigen-specific activation thresholds for T cells. We found that the kinetics of Ca2+ flux in T cells responding to TCR cross-linking was not enhanced in partially activated T cells. Furthermore, following TCR stimulation with anti-cluster of differentiation (CD) 3ε, a lower proportion of partially activated than naive T cells proliferated. In contrast, the proliferation of partially activated and naive ovalbumin peptide (OVAp, SIINFEKL) specific CD8+ T cells (OT-I CD8+ T cells) was similar when stimulated with OVAp. Surprisingly, using an enzyme-linked immunospot (ELISPOT) assay for IFN-γ secretion, we found that a higher number of partially activated OT-I CD8+ T cells expressed effector functions than did naive OT-I CD8+ T cells. This is most readily explained by an increased survival of activated antigen-specific CD8+ T cells from a pool of partially activated T cells than naive T cells. Overall, when examining the effects of early (Ca2+ flux), intermediate (proliferation) or late events (IFN-γ secretion) of T-cell activation, we found that partial activation promotes the survival but does not alter the antigen-specific activation thresholds of CD8+ T cells. | - |
dc.description.statementofresponsibility | Danushka K. Wijesundara, Sheetal Kumar, Mohammed Alsharifi, Arno Müllbacher and Matthias Regner | - |
dc.language.iso | en | - |
dc.publisher | Oxford Univ Press | - |
dc.rights | © The Japanese Society for Immunology. 2010. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1093/intimm/dxq064 | - |
dc.subject | Ca2⁺ | - |
dc.subject | IFN | - |
dc.subject | IFN-γ | - |
dc.subject | r-IFN-β | - |
dc.subject | proliferation | - |
dc.title | Antigen-specific activation thresholds of CD8⁺ T cells are independent of IFN-I-mediated partial lymphocyte activation | - |
dc.title.alternative | Antigen-specific activation thresholds of CD8(+) T cells are independent of IFN-I-mediated partial lymphocyte activation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/intimm/dxq064 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Wijesundara, D. [0000-0002-0740-8362] | - |
Appears in Collections: | Aurora harvest 5 Microbiology and Immunology publications |
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