Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/63359
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Type: Journal article
Title: Comparative assessment of the osteoconductive properties of different biomaterials in vivo seeded with human or ovine mesenchymal stem/stromal cells
Author: Zannettino, A.
Paton, S.
Itescu, S.
Gronthos, S.
Citation: Tissue Engineering. Part A. Tissue Engineering, 2010; 16(12):3579-3587
Publisher: Mary Ann Liebert, Inc. Publishers
Issue Date: 2010
ISSN: 1937-3341
1937-335X
Statement of
Responsibility: 
Andrew C.W. Zannettino, Sharon Paton, Silviu Itescu, Stan Gronthos
Abstract: Mesenchymal stromal/stem cells (MSC), when used in combination with biomaterial scaffolds, have been shown to contribute at varying efficiencies to bone and cartilage regeneration in preclinical large animal models and human clinical trials. In an orthopedic context, identification of the optimal scaffold, which is capable of inducing tissue regeneration, has been the subject of numerous studies. In the present study, we show that ex vivo-expanded MSC from human and ovine bone marrow display similar phenotypic properties, but exhibit differences in their ability to form bone in vivo when transplanted with different biocompatible scaffold composites. We found that the ovine MSC formed ectopic bone on all scaffolds tested with the exception of collagen-based demineralized bone matrix. In contrast, human MSC in general formed less bone and only on those biomaterials composed of ceramic particles containing at least 15% hydroxyapatite. This study demonstrates the differences in bone formation potential between human and ovine MSC in vivo based on the osteoconductive properties of different bioscaffolds currently being used for orthopedic clinical applications.
Keywords: Cells, Cultured; Stromal Cells; Animals; Sheep; Humans; Mice; Mice, SCID; Durapatite; Biocompatible Materials; Flow Cytometry; Materials Testing; Cell Differentiation; Osteogenesis; Adult; Female; Male; Tissue Scaffolds; Young Adult; Mesenchymal Stromal Cells
Rights: © Mary Liebert, Inc.
RMID: 0020101801
DOI: 10.1089/ten.tea.2010.0153
Appears in Collections:Medicine publications

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