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Type: Journal article
Title: Low-frequency spectroscopic analysis of monomeric and fibrillar lysozyme
Author: Zakaria, H.
Fischer, B.
Bradley, A.
Jones, I.
Abbott, D.
Middelberg, A.
Falconer, R.
Citation: Applied Spectroscopy, 2011; 65(3):260-264
Publisher: Soc Applied Spectroscopy
Issue Date: 2011
ISSN: 0003-7028
Statement of
Hidayatul A. Zakaria, Bernd M. Fischer, Andrew P. Bradley, Inke Jones, Derek Abbott, Anton P.J. Middelberg, Robert J. Falconer
Abstract: Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and low-frequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm−1 the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.
Keywords: terahertz
time-domain spectroscopy
fourier transform infrared spectroscopy
FT-IR spectroscopy
vibrational spectroscopy
Rights: Copyright status unknown
DOI: 10.1366/10-06162
Grant ID:
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Electrical and Electronic Engineering publications

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