Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/65609
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Low-frequency spectroscopic analysis of monomeric and fibrillar lysozyme
Author: Zakaria, H.
Fischer, B.
Bradley, A.
Jones, I.
Abbott, D.
Middelberg, A.
Falconer, R.
Citation: Applied Spectroscopy, 2011; 65(3):260-264
Publisher: Soc Applied Spectroscopy
Issue Date: 2011
ISSN: 0003-7028
1943-3530
Statement of
Responsibility: 
Hidayatul A. Zakaria, Bernd M. Fischer, Andrew P. Bradley, Inke Jones, Derek Abbott, Anton P.J. Middelberg, Robert J. Falconer
Abstract: Terahertz time-domain spectroscopy (THz-TDS) and Fourier transform infrared (FT-IR) spectroscopy were used to generate far-infrared and low-frequency spectral measurements of monomeric lysozyme and lysozyme fibrils. The formation of lysozyme fibrils was verified by the Thioflavin T assay and transmission electron microscopy (TEM). It was evident in the FT-IR spectra that between 150 and 350 cm−1 the two spectra diverge, with the lysozyme fibrils showing higher absorbance intensity than the monomeric form. The broad absorption phenomenon is likely due to light scattered from the fibrillar architecture of lysozyme fibrils as supported by simulation of Rayleigh light scattering. The lack of discrete phonon-like peaks suggest that far-infrared spectroscopy cannot detect vibrational modes between the highly ordered hydrogen-bonded beta-pleated sheets of the lysozyme subunit.
Keywords: terahertz; time-domain spectroscopy; THZ-TDS; fourier transform infrared spectroscopy; FT-IR spectroscopy; vibrational spectroscopy; lysozyme; amyloid; fibril
Rights: Copyright status unknown
RMID: 0020103550
DOI: 10.1366/10-06162
Grant ID: http://purl.org/au-research/grants/arc/DP0773111
Appears in Collections:Electrical and Electronic Engineering publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.