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Type: Journal article
Title: Antimicrobial resistance and virulence gene profiles in multi-drug resistant enterotoxigenic Escherichia coli isolated from pigs with post-weaning diarrhoea
Author: Smith, M.
Jordan, D.
Chapman, T.
Chin, J.
Barton, M.
Do, T.
Fahy, V.
Fairbrother, J.
Trott, D.
Citation: Veterinary Microbiology, 2010; 145(3-4):299-307
Publisher: Elsevier Science BV
Issue Date: 2010
ISSN: 0378-1135
Statement of
M.G. Smith, D. Jordan, T.A. Chapman, J.J.-C. Chin, M.D. Barton, T.N. Do, V.A. Fahy, J.M. Fairbrother, D.J. Trott
Abstract: This study aimed to characterize antimicrobial resistance and virulence genes in multi-drug resistant enterotoxigenic Escherichia coli (ETEC) isolates (n=117) collected from porcine post-weaning diarrhoea cases in Australia (1999-2005). Isolates were serotyped, antibiogram-phenotyped for 12 antimicrobial agents and genotyped by PCR for 30 plasmid-mediated antimicrobial resistance genes (ARGs), 22 intestinal and 38 extraintestinal E. coli virulence genes (VGs). Nine serogroups were identified, the most prevalent being O149 (46.2%), O141 (11.2%) and Ont (31.6%). None of the isolates showed resistance to ceftiofur or enrofloxacin and 9.4% were resistant to florfenicol. No corresponding extended-spectrum/AmpC β-lactamase, fluoroquinolone or floR ARGs were detected. An antimicrobial resistance index (ARI) was calculated from the combined data with a weighting for each antimicrobial agent dependent upon its significance to human health. Serogroup O141 isolates had a significantly higher ARI due to an elevated prevalence of aminoglycoside ARGs and possession of more virulence genes (VGs), including ExPEC or EHEC adhesins (bmaE, sfa/focDE, fimH, ihA) in toxin-producing strains that lacked the normally associated F4 and F18 fimbriae. Few associations between ARGs and VGs were apparent, apart from tetC, sfa/focDE and ompT which, for a sub-set of O141 isolates, suggest possible plasmid acquisition from ExPEC. The multi-drug resistant ETEC ARG/VG profiles indicate a high probability of considerable strain and plasmid diversity, reflecting various selection pressures at the individual farm level rather than emergence and lateral spread of MDR resistant/virulent clones.
Keywords: Diarrhoea
Multi-drug resistance
Rights: © 2010 Published by Elsevier B.V.
DOI: 10.1016/j.vetmic.2010.04.004
Appears in Collections:Animal and Veterinary Sciences publications
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