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|Title:||Genetic manipulation of butyrate formation pathways in Clostridium butyricum|
|Citation:||Journal of Biotechnology, 2011; 155(3):269-274|
|Publisher:||Elsevier Science BV|
|Guiqin Cai, Bo Jin, Christopher Saint, Paul Monis|
|Abstract:||Clostridium butyricum is one of the commonly used species for fermentative hydrogen production. While producing H₂, it can produce acids (lactic, acetic and butyric acids) and CO₂, as well as a small amount of ethanol. It has been proposed that elimination of competing pathways, such as the butyrate formation pathway, should increase H₂ yields in Clostridium species. However, the application of this strategy has been hindered by the unavailability of genetic tools for these organisms. In this study, we successfully transferred a plasmid (pMTL007) to C. butyricum by inter-specific conjugation with Escherichia coli and disrupted hbd, the gene encoding β-hydroxybutyryl-CoA dehydrogenase in C. butyricum. Fermentation data showed that inactivation of hbd in C. butyricum eliminated the butyrate formation pathway, resulting in a significant increase in ethanol production and an obvious decrease in H₂ yield compared with the wild type strain. However, under low partial pressure of H₂, the hbd-deficient strain showed increased H₂ production with the simultaneous decrease of ethanol production, indicating that H₂ production by C. butyricum may compete for NADH with the ethanol formation pathway. Together with the discovery of a potential bifurcating hydrogenase, this study extends our understanding of the mechanism of H₂ production by C. butyricum.|
|Keywords:||hbd; Conjugation; Intron integration; Hydrogen production; Bifurcating hydrogenase|
|Rights:||Copyright © 2011 Elsevier B.V. Published by Elsevier B.V. All rights reserved.|
|Appears in Collections:||Earth and Environmental Sciences publications|
Environment Institute publications
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