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https://hdl.handle.net/2440/66768
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Type: | Journal article |
Title: | Genetic manipulation of butyrate formation pathways in Clostridium butyricum |
Author: | Cai, G. Jin, B. Saint, C. Monis, P. |
Citation: | Journal of Biotechnology, 2011; 155(3):269-274 |
Publisher: | Elsevier Science BV |
Issue Date: | 2011 |
ISSN: | 0168-1656 1873-4863 |
Statement of Responsibility: | Guiqin Cai, Bo Jin, Christopher Saint, Paul Monis |
Abstract: | Clostridium butyricum is one of the commonly used species for fermentative hydrogen production. While producing H₂, it can produce acids (lactic, acetic and butyric acids) and CO₂, as well as a small amount of ethanol. It has been proposed that elimination of competing pathways, such as the butyrate formation pathway, should increase H₂ yields in Clostridium species. However, the application of this strategy has been hindered by the unavailability of genetic tools for these organisms. In this study, we successfully transferred a plasmid (pMTL007) to C. butyricum by inter-specific conjugation with Escherichia coli and disrupted hbd, the gene encoding β-hydroxybutyryl-CoA dehydrogenase in C. butyricum. Fermentation data showed that inactivation of hbd in C. butyricum eliminated the butyrate formation pathway, resulting in a significant increase in ethanol production and an obvious decrease in H₂ yield compared with the wild type strain. However, under low partial pressure of H₂, the hbd-deficient strain showed increased H₂ production with the simultaneous decrease of ethanol production, indicating that H₂ production by C. butyricum may compete for NADH with the ethanol formation pathway. Together with the discovery of a potential bifurcating hydrogenase, this study extends our understanding of the mechanism of H₂ production by C. butyricum. |
Keywords: | hbd Conjugation Intron integration Hydrogen production Bifurcating hydrogenase |
Rights: | Copyright © 2011 Elsevier B.V. Published by Elsevier B.V. All rights reserved. |
DOI: | 10.1016/j.jbiotec.2011.07.004 |
Published version: | http://dx.doi.org/10.1016/j.jbiotec.2011.07.004 |
Appears in Collections: | Aurora harvest 5 Earth and Environmental Sciences publications Environment Institute publications |
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