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Type: Journal article
Title: Toward a full characterization of native starch: separation and detection by size-exclusion chromatography
Author: Hoang, N.
Landolfi, A.
Kravchuk, A.
Girard, E.
Peate, J.
Hernandez, J.
Gaborieau, M.
Kravchuk, O.
Gilbert, R.
Guillaneuf, Y.
Castignolles, P.
Citation: Journal of Chromatography A, 2008; 1205(1-2):60-70
Publisher: Elsevier Science BV
Issue Date: 2008
ISSN: 0021-9673
Statement of
Ngoc-Ly Hoang, Antonin Landolfi, Anastasiya Kravchuk, Etienne Girard, Jonathan Peate, Javier M. Hernandez, Marianne Gaborieau, Olena Kravchuk, Robert G. Gilbert, Yohann Guillaneuf and Patrice Castignolles
Abstract: The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.
Keywords: Size-exclusion chromatography (SEC); Separation; Repeatability; Labeling; UV detection; Starch
Rights: Crown Copyright © 2008 Published by Elsevier B.V. All rights reserved.
RMID: 0020114460
DOI: 10.1016/j.chroma.2008.07.090
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Appears in Collections:Agriculture, Food and Wine publications

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