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|Title:||Toward a full characterization of native starch: separation and detection by size-exclusion chromatography|
|Citation:||Journal of Chromatography A, 2008; 1205(1-2):60-70|
|Publisher:||Elsevier Science BV|
|Ngoc-Ly Hoang, Antonin Landolfi, Anastasiya Kravchuk, Etienne Girard, Jonathan Peate, Javier M. Hernandez, Marianne Gaborieau, Olena Kravchuk, Robert G. Gilbert, Yohann Guillaneuf and Patrice Castignolles|
|Abstract:||The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.|
|Keywords:||Size-exclusion chromatography (SEC); Separation; Repeatability; Labeling; UV detection; Starch|
|Rights:||Crown Copyright © 2008 Published by Elsevier B.V. All rights reserved.|
|Appears in Collections:||Agriculture, Food and Wine publications|
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