Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6948
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Type: Journal article
Title: Temporal changes in TFF3 expression and jejunal morphology during methotrexate-induced damage and repair
Author: Xian, C.
Howarth, G.
Mardell, C.
Cool, J.
Familari, M.
Read, L.
Giraud, A.
Citation: American Journal of Physiology - Gastrointestinal and Liver Physiology, 1999; 277(4 40-4):G785-G795
Publisher: AMER PHYSIOLOGICAL SOC
Issue Date: 1999
ISSN: 0193-1857
2163-5773
Abstract: Trefoil factor TFF3 has been implicated in intestinal protection and repair. This study investigated the spatiotemporal relationship between TFF3 expression and morphological changes during intestinal damage and repair in a rat model of methotrexate-induced small intestinal mucositis. Intestinal tissues from rats with mucositis were collected daily for 10 days. Mucosal damage was characterized by an initial decrease in cell proliferation resulting in crypt loss, villus atrophy, and depletion of goblet cells, followed by hyperproliferation that lead to crypt and villus regeneration and mucous cell repopulation. TFF3 mRNA levels increased marginally during histological damage, and the cell population expressing TFF3 mRNA expanded from the usual goblet cells to include some nongoblet epithelial cells before goblet cell repopulation. TFF3 peptide, however, was depleted during histological damage and normalized during repair, mirroring the disappearance and repopulation of goblet cells. Although there is no temporal relationship between TFF3 levels and crypt hyperproliferation, confirming the nonmitogenic nature of TFF3, the coincidental normalization of TFF3 peptide with repopulation of goblet cells and mucin production after proliferative overshoot suggests that TFF3 may play a role in the remodeling phase of repair.
Keywords: Goblet Cells; Jejunum; Animals; Rats; Rats, Sprague-Dawley; Methotrexate; Peptides; Proteins; Muscle Proteins; Mucins; RNA, Messenger; Nucleic Acid Synthesis Inhibitors; Wound Healing; Cell Division; Up-Regulation; Time Factors; Male; Trefoil Factor-3
RMID: 0030005762
DOI: 10.1152/ajpgi.1999.277.4.g785
Appears in Collections:Paediatrics publications

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