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https://hdl.handle.net/2440/7011
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Type: | Journal article |
Title: | Sequence analysis of heparan sulphate and heparin oligosaccharides |
Author: | Vives, R. Pye, D. Salmivirta, M. Hopwood, J. Lindahl, U. Gallagher, J. |
Citation: | Biochemical Journal, 1999; 339(Pt 3):767-773 |
Publisher: | PORTLAND PRESS |
Issue Date: | 1999 |
ISSN: | 0264-6021 1470-8728 |
Statement of Responsibility: | Vivès, Romain R. Pye, David A. ; Salmivirta, Markku ; Hopwood, John J. ; Lindahl, Ulf ; Gallagher, John T. |
Abstract: | The biological activity of heparan sulphate (HS) and heparin largely depends on internal oligosaccharide sequences that provide specific binding sites for an extensive range of proteins. Identification of such structures is crucial for the complete understanding of glycosaminoglycan (GAG)-protein interactions. We describe here a simple method of sequence analysis relying on the specific tagging of the sugar reducing end by 3H radiolabelling, the combination of chemical scission and specific enzymic digestion to generate intermediate fragments, and the analysis of the generated products by strong-anion-exchange HPLC. We present full sequence data on microgram quantities of four unknown oligosaccharides (three HS-derived hexasaccharides and one heparin-derived octasaccharide) which illustrate the utility and relative simplicity of the technique. The results clearly show that it is also possible to read sequences of inhomogeneous preparations. Application of this technique to biologically active oligosaccharides should accelerate progress in the understanding of HS and heparin structure-function relationships and provide new insights into the primary structure of these polysaccharides. |
Keywords: | Mucous Membrane Lysosomes Animals Swine Nitrous Acid Sulfatases Iduronate Sulfatase Iduronidase Heparin Heparitin Sulfate Oligosaccharides Disaccharides Chromatography, High Pressure Liquid Sequence Analysis Binding Sites Carbohydrate Sequence Protein Binding Structure-Activity Relationship Time Factors Molecular Sequence Data |
DOI: | 10.1042/0264-6021:3390767 |
Published version: | http://dx.doi.org/10.1042/0264-6021:3390767 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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