Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/70362
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Cell-specific vacuolar calcium storage mediated by CAX1 regulates apoplastic calcium concentration, gas exchange, and plant productivity in arabidopsis |
Author: | Conn, S. Gilliham, M. Athman, A. Schreiber, A. Baumann, U. Moller, I. Cheng, N. Stancombe, M. Hirschi, K. Webb, A. Burton, R. Kaiser, B. Tyerman, S. Leigh, R. |
Citation: | The Plant Cell, 2011; 23(1):240-257 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2011 |
ISSN: | 1040-4651 1532-298X |
Statement of Responsibility: | Simon J. Conn, Matthew Gilliham, Asmini Athman, Andreas W. Schreiber, Ute Baumann, Isabel Moller, Ning-Hui Cheng, Matthew A. Stancombe, Kendal D. Hirschi, Alex A. R. Webb, Rachel Burton, Brent N. Kaiser, Stephen D. Tyerman, and Roger A. Leigh |
Abstract: | The physiological role and mechanism of nutrient storage within vacuoles of specific cell types is poorly understood. Transcript profiles from Arabidopsis thaliana leaf cells differing in calcium concentration ([Ca], epidermis <10 mM versus mesophyll >60 mM) were compared using a microarray screen and single-cell quantitative PCR. Three tonoplast-localized Ca2+ transporters, CAX1 (Ca2+/H+-antiporter), ACA4, and ACA11 (Ca2+-ATPases), were identified as preferentially expressed in Ca-rich mesophyll. Analysis of respective loss-of-function mutants demonstrated that only a mutant that lacked expression of both CAX1 and CAX3, a gene ectopically expressed in leaves upon knockout of CAX1, had reduced mesophyll [Ca]. Reduced capacity for mesophyll Ca accumulation resulted in reduced cell wall extensibility, stomatal aperture, transpiration, CO2 assimilation, and leaf growth rate; increased transcript abundance of other Ca2+ transporter genes; altered expression of cell wall–modifying proteins, including members of the pectinmethylesterase, expansin, cellulose synthase, and polygalacturonase families; and higher pectin concentrations and thicker cell walls. We demonstrate that these phenotypes result from altered apoplastic free [Ca2+], which is threefold greater in cax1/cax3 than in wild-type plants. We establish CAX1 as a key regulator of apoplastic [Ca2+] through compartmentation into mesophyll vacuoles, a mechanism essential for optimal plant function and productivity. |
Keywords: | Cell Wall Vacuoles Arabidopsis Plant Leaves Calcium Antiporters Cation Transport Proteins Arabidopsis Proteins RNA, Plant Oligonucleotide Array Sequence Analysis Gene Expression Profiling Mutagenesis, Insertional Gene Expression Regulation, Plant Phenotype Mutation Plant Stomata Single-Cell Analysis |
Rights: | © 2011 American Society of Plant Biologists |
DOI: | 10.1105/tpc.109.072769 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1105/tpc.109.072769 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.