Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Full metadata record
DC FieldValueLanguage
dc.contributor.authorConn, S.en
dc.contributor.authorGilliham, M.en
dc.contributor.authorAthman, A.en
dc.contributor.authorSchreiber, A.en
dc.contributor.authorBaumann, U.en
dc.contributor.authorMoller, I.en
dc.contributor.authorCheng, N.en
dc.contributor.authorStancombe, M.en
dc.contributor.authorHirschi, K.en
dc.contributor.authorWebb, A.en
dc.contributor.authorBurton, R.en
dc.contributor.authorKaiser, B.en
dc.contributor.authorTyerman, S.en
dc.contributor.authorLeigh, R.en
dc.identifier.citationPlant Cell, 2011; 23(1):240-257en
dc.description.abstractThe physiological role and mechanism of nutrient storage within vacuoles of specific cell types is poorly understood. Transcript profiles from Arabidopsis thaliana leaf cells differing in calcium concentration ([Ca], epidermis <10 mM versus mesophyll >60 mM) were compared using a microarray screen and single-cell quantitative PCR. Three tonoplast-localized Ca2+ transporters, CAX1 (Ca2+/H+-antiporter), ACA4, and ACA11 (Ca2+-ATPases), were identified as preferentially expressed in Ca-rich mesophyll. Analysis of respective loss-of-function mutants demonstrated that only a mutant that lacked expression of both CAX1 and CAX3, a gene ectopically expressed in leaves upon knockout of CAX1, had reduced mesophyll [Ca]. Reduced capacity for mesophyll Ca accumulation resulted in reduced cell wall extensibility, stomatal aperture, transpiration, CO2 assimilation, and leaf growth rate; increased transcript abundance of other Ca2+ transporter genes; altered expression of cell wall–modifying proteins, including members of the pectinmethylesterase, expansin, cellulose synthase, and polygalacturonase families; and higher pectin concentrations and thicker cell walls. We demonstrate that these phenotypes result from altered apoplastic free [Ca2+], which is threefold greater in cax1/cax3 than in wild-type plants. We establish CAX1 as a key regulator of apoplastic [Ca2+] through compartmentation into mesophyll vacuoles, a mechanism essential for optimal plant function and productivity.en
dc.description.statementofresponsibilitySimon J. Conn, Matthew Gilliham, Asmini Athman, Andreas W. Schreiber, Ute Baumann, Isabel Moller, Ning-Hui Cheng, Matthew A. Stancombe, Kendal D. Hirschi, Alex A. R. Webb, Rachel Burton, Brent N. Kaiser, Stephen D. Tyerman, and Roger A. Leighen
dc.publisherAmer Soc Plant Physiologistsen
dc.rights© 2011 American Society of Plant Biologistsen
dc.subjectCell Wall; Vacuoles; Arabidopsis; Plant Leaves; Calcium; Antiporters; Cation Transport Proteins; Arabidopsis Proteins; RNA, Plant; Oligonucleotide Array Sequence Analysis; Gene Expression Profiling; Mutagenesis, Insertional; Gene Expression Regulation, Plant; Phenotype; Mutation; Plant Stomata; Single-Cell Analysisen
dc.titleCell-specific vacuolar calcium storage mediated by CAX1 regulates apoplastic calcium concentration, gas exchange, and plant productivity in arabidopsisen
dc.typeJournal articleen
pubs.library.collectionAgriculture, Food and Wine publicationsen
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]en
dc.identifier.orcidSchreiber, A. [0000-0002-9081-3405]en
dc.identifier.orcidBaumann, U. [0000-0003-1281-598X]en
dc.identifier.orcidBurton, R. [0000-0002-0638-4709]en
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]en
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.