Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/70362
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dc.contributor.authorConn, S.en
dc.contributor.authorGilliham, M.en
dc.contributor.authorAthman, A.en
dc.contributor.authorSchreiber, A.en
dc.contributor.authorBaumann, U.en
dc.contributor.authorMoller, I.en
dc.contributor.authorCheng, N.en
dc.contributor.authorStancombe, M.en
dc.contributor.authorHirschi, K.en
dc.contributor.authorWebb, A.en
dc.contributor.authorBurton, R.en
dc.contributor.authorKaiser, B.en
dc.contributor.authorTyerman, S.en
dc.contributor.authorLeigh, R.en
dc.date.issued2011en
dc.identifier.citationPlant Cell, 2011; 23(1):240-257en
dc.identifier.issn1040-4651en
dc.identifier.issn1532-298Xen
dc.identifier.urihttp://hdl.handle.net/2440/70362-
dc.description.abstractThe physiological role and mechanism of nutrient storage within vacuoles of specific cell types is poorly understood. Transcript profiles from Arabidopsis thaliana leaf cells differing in calcium concentration ([Ca], epidermis <10 mM versus mesophyll >60 mM) were compared using a microarray screen and single-cell quantitative PCR. Three tonoplast-localized Ca2+ transporters, CAX1 (Ca2+/H+-antiporter), ACA4, and ACA11 (Ca2+-ATPases), were identified as preferentially expressed in Ca-rich mesophyll. Analysis of respective loss-of-function mutants demonstrated that only a mutant that lacked expression of both CAX1 and CAX3, a gene ectopically expressed in leaves upon knockout of CAX1, had reduced mesophyll [Ca]. Reduced capacity for mesophyll Ca accumulation resulted in reduced cell wall extensibility, stomatal aperture, transpiration, CO2 assimilation, and leaf growth rate; increased transcript abundance of other Ca2+ transporter genes; altered expression of cell wall–modifying proteins, including members of the pectinmethylesterase, expansin, cellulose synthase, and polygalacturonase families; and higher pectin concentrations and thicker cell walls. We demonstrate that these phenotypes result from altered apoplastic free [Ca2+], which is threefold greater in cax1/cax3 than in wild-type plants. We establish CAX1 as a key regulator of apoplastic [Ca2+] through compartmentation into mesophyll vacuoles, a mechanism essential for optimal plant function and productivity.en
dc.description.statementofresponsibilitySimon J. Conn, Matthew Gilliham, Asmini Athman, Andreas W. Schreiber, Ute Baumann, Isabel Moller, Ning-Hui Cheng, Matthew A. Stancombe, Kendal D. Hirschi, Alex A. R. Webb, Rachel Burton, Brent N. Kaiser, Stephen D. Tyerman, and Roger A. Leighen
dc.language.isoenen
dc.publisherAmer Soc Plant Physiologistsen
dc.rights© 2011 American Society of Plant Biologistsen
dc.subjectCell Wall; Vacuoles; Arabidopsis; Plant Leaves; Calcium; Antiporters; Cation Transport Proteins; Arabidopsis Proteins; RNA, Plant; Oligonucleotide Array Sequence Analysis; Gene Expression Profiling; Mutagenesis, Insertional; Gene Expression Regulation, Plant; Phenotype; Mutation; Plant Stomata; Single-Cell Analysisen
dc.titleCell-specific vacuolar calcium storage mediated by CAX1 regulates apoplastic calcium concentration, gas exchange, and plant productivity in arabidopsisen
dc.typeJournal articleen
dc.identifier.rmid0020103527en
dc.identifier.doi10.1105/tpc.109.072769en
dc.identifier.pubid31979-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]en
dc.identifier.orcidSchreiber, A. [0000-0002-9081-3405]en
dc.identifier.orcidBaumann, U. [0000-0003-1281-598X]en
dc.identifier.orcidBurton, R. [0000-0002-0638-4709]en
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]en
Appears in Collections:Agriculture, Food and Wine publications

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