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|Title:||Human chromosomal fragile site FRA16B is an amplified AT-rich minisatellite repeat|
Le Paslier, D.
|Citation:||Cell, 1997; 88(3):367-374|
|Yu, Sui; Mangelsdorf, Marie; Hewett, Duncan; Hobson, Lynne; Baker, Elizabeth; Eyre, Helen J; Lapsys, Naras; Le Paslier, Denis; Doggett, Norman A; Sutherland, Grant R; Richards, Robert I|
|Abstract:||Fragile sites are nonstaining gaps in chromosomes induced by specific tissue culture conditions. They vary both in population frequency and in the culture conditions required for induction. Folate-sensitive fragile sites are due to expansion of p(CCG)n trinucleotide repeats; however, the relationship between sequence composition and the chemistry of induction of fragile sites is unclear. To clarify this relationship, the distamycin A-sensitive fragile site FRA16B was isolated by positional cloning and found to be an expanded 33 bp AT-rich minisatellite repeat, p(ATATA TTATATATTATATCTAATAATATATC/ATA)n (consistent with DNA sequence binding preferences of chemicals that induce its cytogenetic expression). Therefore the mutation mechanism associated with trinucleotide repeats is also a property of minisatellite repeats (variable number tandem repeats).|
|Keywords:||Chromosomes, Human, Pair 16; Humans; Chromosome Fragility; DNA, Satellite; Blotting, Southern; Cloning, Molecular; Polymerase Chain Reaction; Gene Amplification; Base Composition; Base Sequence; Minisatellite Repeats; Polymorphism, Genetic; Chromosome Fragile Sites; Molecular Sequence Data|
|Appears in Collections:||Paediatrics publications|
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