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https://hdl.handle.net/2440/7118
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dc.contributor.author | Kumaratilake, L. | - |
dc.contributor.author | Ferrante, A. | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Clinical and Vaccine Immunology, 2000; 7(1):9-13 | - |
dc.identifier.issn | 1071-412X | - |
dc.identifier.issn | 1098-6588 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7118 | - |
dc.description.abstract | A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue. The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1beta significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present. | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.source.uri | http://dx.doi.org/10.1128/cdli.7.1.9-13.2000 | - |
dc.subject | Neutrophils | - |
dc.subject | Phagocytes | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Plasmodium falciparum | - |
dc.subject | Trypan Blue | - |
dc.subject | Fluorescein-5-isothiocyanate | - |
dc.subject | Merozoite Surface Protein 1 | - |
dc.subject | Recombinant Proteins | - |
dc.subject | Antibodies | - |
dc.subject | Cytokines | - |
dc.subject | Flow Cytometry | - |
dc.subject | Phagocytosis | - |
dc.subject | Fluorescence | - |
dc.subject | Time Factors | - |
dc.subject | Software | - |
dc.subject | Melanesia | - |
dc.subject | Papua New Guinea | - |
dc.subject | Opsonin Proteins | - |
dc.subject | Interferon-gamma | - |
dc.title | Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/cdli.7.1.9-13.2000 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ferrante, A. [0000-0002-2581-6407] | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
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