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https://hdl.handle.net/2440/7175
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dc.contributor.author | Han, P. | - |
dc.contributor.author | Story, C. | - |
dc.contributor.author | McDonald, T. | - |
dc.contributor.author | Mrozik, K. | - |
dc.contributor.author | Snell, L. | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Cytotherapy, 2002; 4(2):165-175 | - |
dc.identifier.issn | 1465-3249 | - |
dc.identifier.issn | 1477-2566 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7175 | - |
dc.description.abstract | <h4>Background</h4>Pre-B ALL cells generally elicit a weak immune host response, due to poor expression of co-stimulatory molecules and/or suppression of immune function. A possible way to enhance immunogenicity of pre-B ALL cells is to convert them to DC-like cells.<h4>Methods</h4>To study the effect of ALL cells on T-cell function, ALL cells were incubated with T adult cells activated by OKT3 MAb. Liquid culture of de novo pre-B ALL cells for 7 days, in a medium containing IL-1alpha, IL-3, IL-7, Flt 3 ligand (L) and tumor-necrosis factor alpha (TNF-alpha) produced DC-like cells. These were evaluated for morphology, viability, phenotype, as measured by flow cytometry, and function, including MLR.<h4>Results</h4>Pre-B ALL cell-lines NALM-6, BALM and de novo pre-B ALL cells failed to stimulate T cells, but suppressed stimulated T cells. The DC-like cells displayed characteristic features of DCs: filiform cytoplasmic projections, and phenotypic expression of co-stimulatory molecules CD80/86, MHC Class I and II molecules, CD83 and CD1a. Genetic monoclonality study confirmed their leukemic origin. In a 5-day MLR culture, the DC-like cells potently activated allogeneic adult and cord CD4+ and CD8+ T cells. Furthermore, both CD4+ and CD8+ T cells were primed towards a Type I. No such effect was seen with unmanipulated de novo pre-B ALL cells.<h4>Discussion</h4>DC-like cells can be generated from childhood pre-B ALL cells and are potent stimulators of adult and naïve cord CD8+ T cells via CD4+ cells. These cells may form part of an immunotherapy strategy to overcome tolerance to ALL cells. | - |
dc.description.statementofresponsibility | Han, P ; Story, C ; McDonald, T ; Mrozik, K ; Snell, L | - |
dc.language.iso | en | - |
dc.publisher | Taylor & Francis Ltd | - |
dc.source.uri | http://dx.doi.org/10.1080/146532402317381875 | - |
dc.subject | B-Lymphocytes | - |
dc.subject | Dendritic Cells | - |
dc.subject | T-Lymphocytes | - |
dc.subject | Hematopoietic Stem Cells | - |
dc.subject | Cell Line | - |
dc.subject | Humans | - |
dc.subject | Immunotherapy | - |
dc.subject | Flow Cytometry | - |
dc.subject | Cell Cycle | - |
dc.subject | Cell Differentiation | - |
dc.subject | Precursor Cell Lymphoblastic Leukemia-Lymphoma | - |
dc.title | Immune escape mechanisms of childhood ALL and a potential countering role for DC-like leukemia cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1080/146532402317381875 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Mrozik, K. [0000-0002-4890-8208] | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
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