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Type: Journal article
Title: Construction of a high-resolution physical and transcription map of chromosome 16q24.3 - a region of frequent loss of heterozygosity in sporadic breast cancer
Author: Whitmore, S.
Crawford, J.
Apostolou, S.
Eyre, H.
Baker, E.
Lower, K.
Settasatian, C.
Goldup, S.
Seshadri, R.
Gibson, R.
Mathew, C.
Cleton-Jansen, A.
Savoia, A.
Pronk, J.
Auerbach, A.
Doggett, N.
Sutherland, G.
Callen, D.
Citation: Genomics, 1998; 50(1):1-8
Issue Date: 1998
ISSN: 0888-7543
Statement of
Scott A. Whitmore, Joanna Crawford, Sinoula Apostolou, Helen Eyre, Elizabeth Baker, Karen M. Lower, Chatri Settasatian, Sandra Goldup, Ram Seshadri, Rachel A. Gibson, Christopher G. Mathew, Anne-Marie Cleton-Jansen, Anna Savoia, Jan C. Pronk, Arleen D. Auerbach, Norman A. Doggett, Grant R. Sutherland, David F. Callen
Abstract: A breast cancer tumor suppressor gene has been localized to chromosome 16q24.3 by loss of heterozygosity (LOH) studies of breast tumor DNA. To identify candidate genes for this suppressor function, we have constructed a detailed physical map extending approximately 940 kb from the telomere of the long arm of chromosome 16 that encompasses the minimum LOH interval. This contig consists of a minimum overlapping set of 35 cosmids and a single PAC clone that were aligned by restriction enzyme site mapping. Cosmids were initially identified by screening filters with markers localized to the region by physical mapping using mouse/human somatic cell hybrids, and subsequently cosmid ends were used to complete the contig. A total of seven known genes, including PRSM1, PISSLRE, and the recently cloned Fanconi anemia A (FAA) gene, and potential transcripts from exon-trapping experiments have been located to this contig. A minimum of 14 new transcripts have been identified based on homology of trapped exons with database sequences. This contig and expressed sequence map will form the basis for the identification of the breast cancer tumor suppressor gene in this region.
Keywords: Chromosomes, Human, Pair 16; Humans; Breast Neoplasms; Genetic Markers; In Situ Hybridization, Fluorescence; Physical Chromosome Mapping; Transcription, Genetic; Loss of Heterozygosity; Exons; Molecular Sequence Data
Rights: Copyright © 1998 Academic Press. All rights reserved.
RMID: 0030005638
DOI: 10.1006/geno.1998.5316
Appears in Collections:Paediatrics publications

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