Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73118
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: High-resolution analysis of cytosine methylation in ancient DNA
Author: Llamas, B.
Holland, M.
Chen, K.
Cropley, J.
Cooper, A.
Suter, C.
Citation: PLoS One, 2012; 7(1):1-6
Publisher: Public Library of Science
Issue Date: 2012
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Bastien Llamas, Michelle L. Holland, Kefei Chen, Jennifer E. Cropley, Alan Cooper and Catherine M. Suter
Abstract: Epigenetic changes to gene expression can result in heritable phenotypic characteristics that are not encoded in the DNA itself, but rather by biochemical modifications to the DNA or associated chromatin proteins. Interposed between genes and environment, these epigenetic modifications can be influenced by environmental factors to affect phenotype for multiple generations. This raises the possibility that epigenetic states provide a substrate for natural selection, with the potential to participate in the rapid adaptation of species to changes in environment. Any direct test of this hypothesis would require the ability to measure epigenetic states over evolutionary timescales. Here we describe the first single-base resolution of cytosine methylation patterns in an ancient mammalian genome, by bisulphite allelic sequencing of loci from late Pleistocene Bison priscus remains. Retrotransposons and the differentially methylated regions of imprinted loci displayed methylation patterns identical to those derived from fresh bovine tissue, indicating that methylation patterns are preserved in the ancient DNA. Our findings establish the biochemical stability of methylated cytosines over extensive time frames, and provide the first direct evidence that cytosine methylation patterns are retained in DNA from ancient specimens. The ability to resolve cytosine methylation in ancient DNA provides a powerful means to study the role of epigenetics in evolution.
Keywords: Animals; Mammals; Bison; Cytosine; Retroelements; DNA Methylation; Epigenesis, Genetic; Genome
Rights: Copyright: © 2012 Llamas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020117354
DOI: 10.1371/journal.pone.0030226
Appears in Collections:Earth and Environmental Sciences publications
Environment Institute Leaders publications
Environment Institute publications

Files in This Item:
File Description SizeFormat 
hdl_73118.pdfPublished version809.02 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.