Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7335
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: The Fc receptor for IgG (FcgRII; CD32) on human neonatal B lymphocytes
Author: Jessup, C.
Ridings, J.
Ho, A.
Nobbs, S.
Roberton, D.
Macardle, P.
Zola, H.
Citation: Human Immunology, 2001; 62(7):679-685
Publisher: Elsevier Science Inc
Issue Date: 2001
ISSN: 0198-8859
1879-1166
Abstract: B cells express an Fc receptor for IgG (FcgammaRII; CD32) which is involved in feedback inhibition of antibody production. Engagement of FcgammaRII during ligation of the antigen receptor provides an inhibitory signal. FcgammaRII exists as several isoforms, with FcgammaRIIb (which carries an immunoreceptor tyrosine-based inhibition motif; ITIM) being predominant form on adult B cells. The inhibitory role of FcgammaRIIb may be unhelpful to the infant, since primary exposure to infectious agents is likely to be in the presence of maternal IgG. We hypothesized that neonatal B cells would be less susceptible to feedback inhibition by antibody, either through the expression of activation-competent FcgammaRII isoforms (FcgammaRIIa and FcgammaRIIc) or through reduced expression of the inhibitory FcgammaRIIb isoforms. Cord and adult B cells were examined for expression of FcgammaRII isoforms using monoclonal antibodies and RT-PCR. In vitro assays were performed to assess susceptibility of cord and adult cells to FcgammaRII-mediated suppression. Although there is no phenotypic difference in FcgammaRII expression (FcgammaRIIb predominating on both adult and cord B cells), FcgammaRIIb is expressed at lower levels on cord cells. This quantitative difference in FcgammaRIIb expression may explain the reduced susceptibility of cord B cells to antibody-mediated inhibition observed in these experiments.
Keywords: B-Lymphocyte Subsets; Fetal Blood; Humans; Growth Inhibitors; Immunoglobulin M; Receptors, IgG; Protein Isoforms; Immunosuppressive Agents; Antibodies, Anti-Idiotypic; Antibodies, Monoclonal; Reverse Transcriptase Polymerase Chain Reaction; Lymphocyte Activation; Adult; Infant, Newborn; Immunoglobulin Fab Fragments
RMID: 0020010910
DOI: 10.1016/S0198-8859(01)00257-9
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.