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|Title:||Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli|
|Citation:||Journal of Infectious Diseases, 2012; 206(8):1242-1249|
|Publisher:||University of Chicago Press|
|Rebecca E. Watts, Chee K. Tan, Glen C. Ulett, Alison J. Carey, Makrina Totsika, Adi Idris, Adrienne W. Paton, Renato Morona, James C. Paton, and Mark A. Schembri|
|Abstract:||Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to α-D-galactopyranosyl-(1-4)-β-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972:: lgtCE, impaired P fimbriae–mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.|
|Keywords:||Erythrocytes; Epithelial Cells; Animals; Mice, Inbred C57BL; Humans; Mice; Urinary Tract Infections; Disease Models, Animal; Oligosaccharides; Fimbriae Proteins; Bacterial Adhesion; Protein Binding; Female; Uropathogenic Escherichia coli|
|Rights:||© The Author 2012.|
|Appears in Collections:||Microbiology and Immunology publications|
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