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Type: Journal article
Title: Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli
Author: Watts, R.
Tan, C.
Ulett, G.
Carey, A.
Totsika, M.
Idris, A.
Paton, A.
Morona, R.
Paton, J.
Schembri, M.
Citation: Journal of Infectious Diseases, 2012; 206(8):1242-1249
Publisher: University of Chicago Press
Issue Date: 2012
ISSN: 0022-1899
Statement of
Rebecca E. Watts, Chee K. Tan, Glen C. Ulett, Alison J. Carey, Makrina Totsika, Adi Idris, Adrienne W. Paton, Renato Morona, James C. Paton, and Mark A. Schembri
Abstract: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are a significant health concern, exacerbated by the rapid emergence of multidrug resistant strains refractory to antibiotic treatment. P fimbriae are strongly associated with upper urinary tract colonization due to specific binding to α-D-galactopyranosyl-(1-4)-β-D-galactopyranoside receptors in the kidneys. Thus, inhibiting P-fimbrial adhesion may reduce the incidence of UPEC-mediated UTI. E. coli 83972 is an asymptomatic bacteriuria isolate successfully used as a prophylactic agent to prevent UTI in human studies. We constructed a recombinant E. coli 83972 strain displaying a surface-located oligosaccharide P fimbriae receptor mimic that bound to P-fimbriated E. coli producing any of the 3 PapG adhesin variants. The recombinant strain, E. coli 83972:: lgtCE, impaired P fimbriae–mediated adhesion to human erythrocytes and kidney epithelial cells. Additionally, E. coli 83972::lgtCE impaired urine colonization by UPEC in a mouse UTI model, demonstrating its potential as a prophylactic agent to prevent UTI.
Keywords: Erythrocytes; Epithelial Cells; Animals; Mice, Inbred C57BL; Humans; Mice; Urinary Tract Infections; Disease Models, Animal; Oligosaccharides; Fimbriae Proteins; Bacterial Adhesion; Protein Binding; Female; Uropathogenic Escherichia coli
Rights: © The Author 2012.
RMID: 0020122013
DOI: 10.1093/infdis/jis493
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Appears in Collections:Microbiology and Immunology publications
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