Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/73637
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Type: Journal article
Title: Heparan sulfate proteoglycans regulate responses to oocyte paracrine signals in ovarian follicle morphogenesis
Author: Watson, L.
Mottershead, D.
Dunning, K.
Robker, R.
Gilchrist, R.
Russell, D.
Citation: Endocrinology, 2012; 153(9):4544-4555
Publisher: Endocrine Soc
Issue Date: 2012
ISSN: 0013-7227
1945-7170
Statement of
Responsibility: 
Laura N. Watson, David G. Mottershead, Kylie R. Dunning, Rebecca L. Robker, Robert B. Gilchrist and Darryl L. Russell
Abstract: In the ovarian follicle, oocyte-secreted factors induce cumulus-specific genes and repress mural granulosa cell specific genes to establish these functionally distinct cell lineages. The mechanism establishing this precise morphogenic pattern of oocyte signaling within the follicle is unknown. The present study investigated a role for heparan sulphate proteoglycans (HSPG) as coreceptors mediating oocyte secreted factor signaling. In vitro maturation of cumulus oocyte complexes in the presence of exogenous heparin, which antagonizes HSPG signaling, prevented cumulus expansion and blocked the induction of cumulus-specific matrix genes, Has2 and Tnfaip6, whereas conversely, the mural granulosa-specific genes, Lhcgr and Cyp11a1, were strongly up-regulated. Heparin also blocked phosphorylation of SMAD2. Exogenous growth differentiation factor (GDF)-9 reversed these heparin effects; furthermore, GDF9 strongly bound to heparin sepharose. These observations indicate that heparin binds endogenous GDF9 and disrupts interaction with heparan sulphate proteoglycan coreceptor(s), important for GDF9 signaling. The expression of candidate HSPG coreceptors, Syndecan 1–4, Glypican 1–6, and Betaglycan, was examined. An ovulatory dose of human chorionic gonadotropin down-regulated Betaglycan in cumulus cells, and this regulation required GDF9 activity; conversely, Betaglycan was significantly increased in luteinizing mural granulosa cells. Human chorionic gonadotropin caused very strong induction of Syndecan 1 and Syndecan 4 in mural granulosa as well as cumulus cells. Glypican 1 was selectively induced in cumulus cells, and this expression appeared dependent on GDF9 action. These data suggest that HSPG play an essential role in GDF9 signaling and are involved in the patterning of oocyte signaling and cumulus cell function in the periovulatory follicle.
Keywords: Ovarian Follicle
Oocytes
Animals
Mice
Benzamides
Dioxoles
Polymerase Chain Reaction
Morphogenesis
Female
Heparan Sulfate Proteoglycans
Growth Differentiation Factor 9
Rights: Copyright © 2012 by The Endocrine Society
DOI: 10.1210/en.2012-1181
Grant ID: http://purl.org/au-research/grants/nhmrc/519235
http://purl.org/au-research/grants/nhmrc/1011297
ARC
Appears in Collections:Aurora harvest 5
Obstetrics and Gynaecology publications

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