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https://hdl.handle.net/2440/7380
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Type: | Journal article |
Title: | Direct evidence that ERK regulates the production/secretion of interleukin-2 in PHA/PMA-stimulated T lymphocytes |
Author: | Li, Y. Hii, C. Der, C. Ferrante, A. |
Citation: | Immunology, 1999; 96(4):524-528 |
Publisher: | BLACKWELL SCIENCE LTD |
Issue Date: | 1999 |
ISSN: | 0019-2805 1365-2567 |
Abstract: | Although p21ras, raf-1 and MEK have been shown to regulate directly the transcriptional activity of NFAT (nuclear factor of activated T cells) and/or the interleukin-2 (IL-2) promoter, direct evidence that the extracellular signal-regulated protein kinase (ERK) is involved in regulating IL-2 production is still lacking. Here, we demonstrate that transfection of Jurkat cells with a dominant negative mutant of ERK1 (Erk1-K71R) resulted in the suppression of mitogen-stimulated production/secretion of IL-2. This was accompanied by a parallel inhibition of mitogen-stimulated ERK activity. These data provide direct evidence, for the first time, that ERK plays a vital role in regulating the production/secretion of IL-2. |
Keywords: | T-Lymphocytes Tumor Cells, Cultured Humans Tetradecanoylphorbol Acetate Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase 3 Phytohemagglutinins Interleukin-2 Blotting, Western Transfection Lymphocyte Activation Calcium-Calmodulin-Dependent Protein Kinases |
DOI: | 10.1046/j.1365-2567.1999.00724.x |
Published version: | http://dx.doi.org/10.1046/j.1365-2567.1999.00724.x |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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