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|Title:||Legionella steelei sp. nov., isolated from human respiratory specimens in California, USA, and South Australia|
|Citation:||International Journal of Systematic and Evolutionary Microbiology, 2012; 62(8):1766-1771|
|Publisher:||Soc General Microbiology|
|Paul H. Edelstein, Martha A. Edelstein, Lisa J. Shephard, Kevin W. Ward and Rodney M. Ratcliff.|
|Abstract:||Legionella-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376T, were found to have identical Legionella macrophage infectivity potentiator (mip) gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue–white fluorescent pigment. The optimal in vitro growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in Acanthamoeba castellani at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, mip, ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus Legionella for which the name Legionella steelei sp. nov. is proposed. The type strain is IMVS-3376T ( = IMVS 3113T = ATCC BAA-2169T).|
|Keywords:||Respiratory System; Cell Line; Humans; Legionella; DNA, Bacterial; RNA, Ribosomal, 16S; Pigmentation; Sequence Analysis, DNA; Temperature; Phylogeny; Genotype; Molecular Sequence Data; Adult; California; South Australia; Male|
|Description:||The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mip, proA, rnpB and rpoB gene sequences of strain IMVS-3376T are HQ398202–HQ398206, respectively.|
|Rights:||Copyright © 2012 International Union of Microbiological Societies|
|Appears in Collections:||Microbiology and Immunology publications|
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