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https://hdl.handle.net/2440/74144
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Type: | Journal article |
Title: | Legionella steelei sp. nov., isolated from human respiratory specimens in California, USA, and South Australia |
Author: | Edelstein, P. Edelstein, M. Shephard, L. Ward, K. Ratcliff, R. |
Citation: | International Journal of Systematic and Evolutionary Microbiology, 2012; 62(8):1766-1771 |
Publisher: | Soc General Microbiology |
Issue Date: | 2012 |
ISSN: | 1466-5026 1466-5034 |
Statement of Responsibility: | Paul H. Edelstein, Martha A. Edelstein, Lisa J. Shephard, Kevin W. Ward and Rodney M. Ratcliff. |
Abstract: | Legionella-like bacteria were isolated from the respiratory tract of two patients in California, USA, and South Australia, but were not thought to cause disease. These bacteria, strains F2632 and IMVS-3376T, were found to have identical Legionella macrophage infectivity potentiator (mip) gene sequences and were therefore further characterized to determine their genetic and phenotypic relatedness and properties. Both of these Gram-negative-staining bacterial strains grew on buffered charcoal yeast extract medium, were cysteine auxotrophs and made a characteristic diffusible bright yellow fluorescent pigment, with one strain making a late appearing colony-bound blue–white fluorescent pigment. The optimal in vitro growth temperature was 35 °C, with very poor growth at 37 °C in broth or on solid media. There was no growth in human A549 cells at either 35 or 37 °C, but excellent growth in Acanthamoeba castellani at 30 °C and poorer growth at 35 °C. Phylogenetic analysis of these bacteria was performed by sequence analysis of 16S rRNA, mip, ribonuclease P, ribosomal polymerase B and zinc metalloprotease genes. These studies confirmed that the new strains represented a single novel species of the genus Legionella for which the name Legionella steelei sp. nov. is proposed. The type strain is IMVS-3376T ( = IMVS 3113T = ATCC BAA-2169T). |
Keywords: | Respiratory System Cell Line Humans Legionella DNA, Bacterial RNA, Ribosomal, 16S Pigmentation Sequence Analysis, DNA Temperature Phylogeny Genotype Molecular Sequence Data Adult California South Australia Male |
Description: | The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, mip, proA, rnpB and rpoB gene sequences of strain IMVS-3376T are HQ398202–HQ398206, respectively. |
Rights: | Copyright © 2012 International Union of Microbiological Societies |
DOI: | 10.1099/ijs.0.035709-0 |
Published version: | http://dx.doi.org/10.1099/ijs.0.035709-0 |
Appears in Collections: | Aurora harvest Microbiology and Immunology publications |
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