Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7434
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Type: Journal article
Title: HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor
Author: Lalonde, J.
Lim, R.
Ingley, E.
Tilbrook, P.
Thompson, M.
McCulloch, R.
Beaumont, J.
Wicking, C.
Eyre, H.
Sutherland, G.
Howe, K.
Solomon, E.
Williams, J.
Klinken, S.
Citation: Journal of Biological Chemistry, 2004; 279(9):8181-8189
Publisher: Amer Soc Biochemistry Molecular Biology Inc
Issue Date: 2004
ISSN: 0021-9258
1083-351X
Statement of
Responsibility: 
Jean-Philippe Lalonde, Raelene Lim, Evan Ingley, Peta A. Tilbrook, Martin J. Thompson, Ross McCulloch, Jennifer G. Beaumont, Carol Wicking, Helen J. Eyre, Grant R. Sutherland, Kathy Howe, Ellen Solomon, James H. Williams, and S. Peter Klinken
Abstract: Hemopoietic cells, apparently committed to one lineage, can be reprogrammed to display the phenotype of another lineage. The J2E erythroleukemic cell line has on rare occasions developed the features of monocytic cells. Subtractive hybridization was used in an attempt to identify genes that were up-regulated during this erythroid to myeloid transition. We report here on the isolation of hemopoietic lineage switch 5 (Hls5), a gene expressed by the monocytoid variant cells, but not the parental J2E cells. Hls5 is a novel member of the RBCC (Ring finger, B box, coiled-coil) family of genes, which includes Pml, Herf1, Tif-1alpha, and Rfp. Hls5 was expressed in a wide range of adult tissues; however, at different stages during embryogenesis, Hls5 was detected in the branchial arches, spinal cord, dorsal root ganglia, limb buds, and brain. The protein was present in cytoplasmic granules and punctate nuclear bodies. Isolation of the human cDNA and genomic DNA revealed that the gene was located on chromosome 8p21, a region implicated in numerous leukemias and solid tumors. Enforced expression of Hls5 in HeLa cells inhibited cell growth, clonogenicity, and tumorigenicity. It is conceivable that HLS5 is one of the tumor suppressor genes thought to reside at the 8p21 locus.
Keywords: Extremities; Brain; Spinal Cord; Ganglia, Spinal; Hematopoietic Stem Cells; Cell Line, Tumor; Hela Cells; Chromosomes, Human, Pair 8; Cell Nucleus; Cytoplasmic Granules; Branchial Region; Animals; Humans; Mice; Leukemia, Erythroblastic, Acute; Carrier Proteins; DNA; DNA, Complementary; Microscopy, Fluorescence; Transfection; Cell Cycle; Apoptosis; Cell Differentiation; Brain Chemistry; Amino Acid Sequence; Base Sequence; Embryonic and Fetal Development; Genes, Tumor Suppressor; Open Reading Frames; Molecular Sequence Data; Apoptosis Regulatory Proteins
RMID: 0020040145
DOI: 10.1074/jbc.M306751200
Appears in Collections:Paediatrics publications

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