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Type: Journal article
Title: Improved efficiency of doubled haploid generation in hexaploid triticale by in vitro chromosome doubling
Author: Wurschum, T.
Tucker, M.
Reif, J.
Maurer, H.
Citation: BMC Plant Biology, 2012; 12(1):1-6
Publisher: BioMed Central Ltd.
Issue Date: 2012
ISSN: 1471-2229
Statement of
Tobias Würschum, Matthew R Tucker, Jochen C Reif and Hans Peter Maurer
Abstract: Background: Doubled haploid production is a key technology in triticale research and breeding. A critical component of this method depends on chromosome doubling, which is traditionally achieved by in vivo treatment of seedlings with colchicine. Results: In this study we investigated the applicability of an in vitro approach for chromosome doubling based on microspore culture. Our results show a pronounced increase in the proportion of doubled haploid triticale plants compared to the spontaneous doubling rate, but also compared to the doubling obtained by the standard in vivo approach. In addition, the frequency of plants surviving from culture medium to maturity is also much higher for the in vitro approach. Colchicine concentrations of 1 mM for 24 h or 0.3 mM applied for 48 or 72 h during the first hours of microspore culture performed best. Conclusions: Our results suggest that for triticale, in vitro chromosome doubling is a promising alternative to the in vivo approach.
Keywords: Chromosomes, Plant; Cereals; Colchicine; Tissue Culture Techniques; Breeding; Haploidy; Polyploidy; Time Factors
Description: Extent: 7p.
Rights: © 2012 Würschum et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020122090
DOI: 10.1186/1471-2229-12-109
Appears in Collections:Agriculture, Food and Wine publications

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