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https://hdl.handle.net/2440/7449
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Type: | Journal article |
Title: | Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9 |
Author: | Wang, Z. Qiu, Q. Seufert, W. Taguchi, T. Testa, J. Whitmore, S. Callen, D. Welsh, D. Shenk, T. Deuel, T. |
Citation: | Journal of Biological Chemistry, 1996; 271(40):24811-24816 |
Publisher: | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
Issue Date: | 1996 |
ISSN: | 0021-9258 1083-351X |
Abstract: | We report a novel human gene whose product specifically associates with the negative regulatory domain of the Wilms' tumor gene product (WT1) in a yeast two-hybrid screen and with WT1 in immunoprecipitation and glutathione S-transferase (GST) capture assays. The gene encodes a 17-kDa protein that has 56% amino acid sequence identity with yeast ubiquitin-conjugating enzyme (yUBC) 9, a protein required for cell cycle progression in yeast, and significant identity with other subfamilies of ubiquitin-conjugating enzymes. The human gene fully complements yeast that have a temperature-sensitive yUBC9 gene mutation to fully restore normal growth, indicating that we have cloned a functionally conserved human (h) homolog of yUBC9. Transcripts of hUBC9 of 4.4 kilobases (kb), 2.8 kb, and 1.3 kb were found in all human tissues tested. A single copy of the hUBC9 gene was found and localized to human chromosome 16p13.3. We conclude that hUBC9 retains striking structural and functional conservation with yUBC9 and suggest a possible link of the ubiquitin/proteosome proteolytic pathway and the WT1 transcriptional repressor system. |
Keywords: | Chromosomes, Human, Pair 16 Humans Ligases Ubiquitin-Conjugating Enzymes DNA-Binding Proteins WT1 Proteins Transcription Factors DNA, Complementary Chromosome Mapping Cloning, Molecular Amino Acid Sequence Protein Binding Sequence Homology, Amino Acid Molecular Sequence Data |
DOI: | 10.1074/jbc.271.40.24811 |
Published version: | http://dx.doi.org/10.1074/jbc.271.40.24811 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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