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https://hdl.handle.net/2440/75549
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Type: | Journal article |
Title: | Microsatellites from rubber tree (Hevea brasiliensis) for genetic diversity analysis and cross-amplification in six Hevea wild species |
Author: | Souza, L. Mantello, C. de Oliveira Santos Garcia, M. de Souza Goncalves, P. Souza, A. |
Citation: | Conservation Genetics Resources, 2009; 1(1):75-79 |
Publisher: | Springer Netherlands |
Issue Date: | 2009 |
ISSN: | 1877-7252 1877-7260 |
Statement of Responsibility: | L. M. Souza, C. C. Mantello, M. O. Santos, P. de Souza Gonçalves, Anete Pereira Souza |
Abstract: | Hevea brasiliensis is native to the Amazonian rain forest and an important source of natural rubber. Twenty seven polymorphic microsatellite loci were isolated and characterized from a GA-CA enriched genomic library of H. brasiliensis. The number of alleles ranged from 2 to 20. The observed and expected heterozygosity ranged from 0.13 to 0.88 and from 0.00 to 0.89, respectively. Cross-species amplification of the markers developed for H. brasiliensis was successful in the wild Hevea species H. guianensis, H. rigidifolia, H. nitida, H. pauciflora, H. benthamiana and H. camargoana. The data indicated a high degree of sequence homology in the microsatellite flanking regions of these species. The developed SSR loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow among Hevea species. © Springer Science+Business Media B.V. 2009. |
Keywords: | Hevea spp. Hevea brasiliensis Microsatellite Genetic diversity Transferability Cross-species amplification |
Rights: | © Springer Science+Business Media B.V. 2009 |
DOI: | 10.1007/s12686-009-9018-7 |
Published version: | http://dx.doi.org/10.1007/s12686-009-9018-7 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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