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Type: Journal article
Title: Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers
Author: Ferreirs-Ramos, R.
Laborda, P.
de Oliveira Santos Garcia, M.
Mayor, M.
Mestriner, M.
Souza, A.
Alzate-Marin, A.
Citation: Conservation Genetics, 2007; 9(5):1281-1285
Publisher: Springer-Verlag Dordrecht
Issue Date: 2007
ISSN: 1566-0621
Statement of
Ronai Ferreira-Ramos, Prianda R. Laborda, Melissa de Oliveira Santos, Matheus S. Mayor, Moacyr A. Mestriner, Anete P. de Souza, Ana Lilia Alzate-Marin
Abstract: Nine microsatellite loci for genetic analysis of three populations of the tropical tree Eugenia uniflora L. (pitanga or Brazilian cherry) from fragments of semideciduous forest were developed. We used the technique of building a (GA) n and (CA) n microsatellite-enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 84 mature trees found in three areas (Ribeirão Preto, Tambaú and São José do Rio Pardo), highly impacted by the agricultural practices, in a large region among Pardo river and Mogi-Guaçu river basins, in state of São Paulo, Brazil. All loci were polymorphic, and the number of alleles was high, ranging from 6 to 24, with a mean of 14.4. All stands showed the same high level of genetic diversity (mean H E = 0.83) and a low genetic differentiation (mean F ST = 0.031), indicating that genetic diversity was higher within rather than among populations. Seven of the nine loci were highly variable, and sufficiently informative for E. uniflora. It was concluded that these new SSR markers can be efficiently used for gene flow studies.
Keywords: Conservation; Development; Forest; Genetic diversity; Myrtaceae; Pitanga
Rights: © Springer Science+Business Media B.V. 2007
RMID: 0020111871
DOI: 10.1007/s10592-007-9458-0
Appears in Collections:Agriculture, Food and Wine publications

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