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https://hdl.handle.net/2440/76868
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dc.contributor.author | Wong, M.X. | - |
dc.contributor.author | Roberts, D. | - |
dc.contributor.author | Bartley, P. | - |
dc.contributor.author | Jackson, D. | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Journal of Immunology, 2002; 168(12):6455-6462 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.issn | 1550-6606 | - |
dc.identifier.uri | http://hdl.handle.net/2440/76868 | - |
dc.description.abstract | Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a newly assigned member of the Ig-immunoreceptor tyrosine-based inhibitory motif superfamily, and its functional role is suggested to be an inhibitory receptor that modulates immunoreceptor tyrosine-based activation motif-dependent signaling cascades. In this study, we hypothesized that PECAM-1 plays an essential in vivo role as a counterregulator of immediate hypersensitivity reactions. We found that PECAM-1 was highly expressed on the surface of immature bone marrow mast cells and at a lower density on mature peritoneal mast cells. Examination of skin biopsies from PECAM-1(+/+) and PECAM-1(-/-) mice revealed that absence of PECAM-1 did not affect mast cell development or the capacity of mast cells to populate tissues. To examine whether the absence of PECAM-1 would influence immediate hypersensitivity reactions, PECAM-1(+/+) and PECAM-1(-/-) mice were presensitized with anti-DNP mouse IgE and then challenged 20 h later with DNP-BSA or PBS. PECAM-1(-/-) mice exhibited elevated serum histamine concentrations after Ag stimulation compared with PECAM-1(+/+) mice, indicating an increased severity of systemic IgE-mediated anaphylaxis. PECAM-1(-/-) mice have increased sensitivity to local cutaneous IgE-dependent anaphylaxis compared with PECAM-1(+/+) mice, as assessed by greater tissue swelling of their ears and mast cell degranulation in situ. PECAM-1(-/-) bone marrow mast cells showed enhanced dense granule serotonin release after Fc epsilon RI cross-linking in vitro. These results suggest that PECAM-1 acts as a counterregulator in allergic disease susceptibility and severity and negatively modulates mast cell activation. | - |
dc.description.statementofresponsibility | Mae-Xhum Wong, Donna Roberts, Paul A. Bartley, and Denise E. Jackson | - |
dc.description.uri | http://jimmunol.org/content/168/12/6455 | - |
dc.language.iso | en | - |
dc.publisher | American Association of Immunologists | - |
dc.rights | Copyright © 2002 by The American Association of Immunologists | - |
dc.source.uri | http://dx.doi.org/10.4049/jimmunol.168.12.6455 | - |
dc.subject | Bone Marrow Cells | - |
dc.subject | Cells, Cultured | - |
dc.subject | Mast Cells | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred C57BL | - |
dc.subject | Mice, Knockout | - |
dc.subject | Mice | - |
dc.subject | Anaphylaxis | - |
dc.subject | Disease Susceptibility | - |
dc.subject | Edema | - |
dc.subject | Immunoglobulin E | - |
dc.subject | Receptors, IgE | - |
dc.subject | Passive Cutaneous Anaphylaxis | - |
dc.subject | Severity of Illness Index | - |
dc.subject | Cell Differentiation | - |
dc.subject | Cell Degranulation | - |
dc.subject | Organ Specificity | - |
dc.subject | Up-Regulation | - |
dc.subject | Platelet Endothelial Cell Adhesion Molecule-1 | - |
dc.title | Absence of platelet endothelial cell adhesion molecule-1 (CD31) leads to increased severity of local and systemic IgE-mediated anaphylaxis and modulation of mast cell activation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.4049/jimmunol.168.12.6455 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Roberts, D. [0000-0002-8292-2142] | - |
Appears in Collections: | Aurora harvest Psychology publications |
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