Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/77470
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Type: Journal article
Title: A comprehensive expression profile of microRNAs and other classes of non-coding small RNAs in barley under phosphorous-deficient and -sufficient conditions
Author: Hackenberg, M.
Huang, P.
Huang, C.
Shi, B.
Gustafson, P.
Langridge, P.
Citation: DNA Research, 2013; 20(2):109-125
Publisher: Universal Academy Press Inc
Issue Date: 2013
ISSN: 1340-2838
1756-1663
Statement of
Responsibility: 
Michael Hackenberg, Po-Jung Huang, Chun-Yuan Huang, Bu-Jun Shi, Perry Gustafson and Peter Langridge
Abstract: Phosphorus (P) is essential for plant growth. MicroRNAs (miRNAs) play a key role in phosphate homeostasis. However, little is known about P effect on miRNA expression in barley (Hordeum vulgare L.). In this study, we used Illumina's next-generation sequencing technology to sequence small RNAs (sRNAs) in barley grown under P-deficient and P-sufficient conditions. We identified 221 conserved miRNAs and 12 novel miRNAs, of which 55 were only present in P-deficient treatment while 32 only existed in P-sufficient treatment. Total 47 miRNAs were significantly differentially expressed between the two P treatments (|log₂| > 1). We also identified many other classes of sRNAs, including sense and antisense sRNAs, repeat-associated sRNAs, transfer RNA (tRNA)-derived sRNAs and chloroplast-derived sRNAs, and some of which were also significantly differentially expressed between the two P treatments. Of all the sRNAs identified, antisense sRNAs were the most abundant sRNA class in both P treatments. Surprisingly, about one-fourth of sRNAs were derived from the chloroplast genome, and a chloroplast-encoded tRNA-derived sRNA was the most abundant sRNA of all the sRNAs sequenced. Our data provide valuable clues for understanding the properties of sRNAs and new insights into the potential roles of miRNAs and other classes of sRNAs in the control of phosphate homeostasis.
Keywords: deep sequencing; barley; phosphorus; non-coding small RNAs; miRNAs; differential expression
Rights: © The Author 2012. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020127011
DOI: 10.1093/dnares/dss037
Appears in Collections:Agriculture, Food and Wine publications

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