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https://hdl.handle.net/2440/7771
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Type: | Journal article |
Title: | Detection and significance of the potentially pathogenic amoeboflagellate Naegleria italica in Australia |
Author: | Robinson, B. Monis, P. Henderson, M. Gelonese, S. Ferrante, A. |
Citation: | Parasitology International, 2004; 53(1):23-27 |
Publisher: | Elsevier BV |
Issue Date: | 2004 |
ISSN: | 1383-5769 1873-0329 |
Statement of Responsibility: | Bret S. Robinson, Paul T. Monis, Mandy Henderson, Stella Gelonese, Antonio Ferrante |
Abstract: | Thermophilic amoeboflagellates in the genus Naegleria include both virulent and benign species. One of the less studied species, N. italica, has not been detected in the environment since the first reports from Italy in the 1980s; its virulence is known only from infection of laboratory mice. Two recent strains from recreational water in Western Australia (AWQC NG960, NG961) were tentatively identified as N. italica from the characteristic mobilities of seven isozymes. Sequences of the 5.8S rRNA gene and its flanking ITS aligned with a 380+bp length of the published sequence for N. italica with 98% identity. Differences from the type strain were confined to ITS2. Shorter alignments (<320 bp) were observed with other Naegleria species, corresponding to conserved regions of the 5.8S gene and ITS. Unlike the European type strain of N. italica, the Australian isolates failed to infect laboratory mice intranasally, confirming that infectivity of this species is variable and often lower than in N. fowleri. |
Keywords: | Animals Mice, Inbred BALB C Mice Naegleria Amebiasis Water DNA, Ribosomal Spacer DNA, Protozoan RNA, Ribosomal, 5.8S Sequence Analysis, DNA Virulence Molecular Sequence Data Western Australia Female |
DOI: | 10.1016/j.parint.2003.10.002 |
Published version: | http://dx.doi.org/10.1016/j.parint.2003.10.002 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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