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Type: Journal article
Title: Immunolocalization of NLRP3 inflammasome in normal murine airway epithelium and changes following induction of ovalbumin-induced airway inflammation
Author: Tran, H.
Lewis, M.
Tan, L.W.
Lester, S.
Baker, L.
Ng, J.
Hamilton-Bruce, M.
Hill, C.
Koblar, S.
Rischmueller, M.
Ruffin, R.
Wormald, P.J.
Zalewski, P.
Lang, C.
Citation: Journal of Allergy, 2012; Online(819176):1-13
Publisher: Hindawi Publishing Corporation
Issue Date: 2012
ISSN: 1687-9791
Statement of
Hai B. Tran, Martin D. Lewis, LorWai Tan, Susan E. Lester, Leonie M. Baker, Jia Ng, Monica A. Hamilton-Bruce, Catherine L. Hill, Simon A. Koblar, Maureen Rischmueller, Richard E. Ruffin, Peter J. Wormald, Peter D. Zalewski, and Carol J. Lang
Abstract: Little is known about innate immunity and components of inflammasomes in airway epithelium. This study evaluated immunohistological evidence for NLRP3 inflammasomes in normal and inflamed murine (Balb/c) airway epithelium in a model of ovalbumin (OVA) induced allergic airway inflammation. The airway epithelium of control mice exhibited strong cytoplasmic staining for total caspase-1, ASC, and NLRP3, whereas the OVA mice exhibited strong staining for active caspase-1, with redistribution of caspase-1, IL-1β and IL-18, indicating possible activation of the NLRP3 inflammasome. Active caspase-1, NLRP3, and other inflammasome components were also detected in tissue eosinophils from OVA mice, and may potentially contribute to IL-1β and IL-18 production. In whole lung, inRNA expression of NAIP and procaspase-1 was increased in OVA mice, whereas NLRP3, IL-1β and IL-18 decreased. Some OVA-treated mice also had significantly elevated and tightly correlated serum levels of IL-1β and TNFα. In cultured normal human bronchial epithelial cells, LPS priming resulted in a significant increase in NLRP3 and II-lp protein expression. This study is the first to demonstrate NLRP3 inflammasome components in normal airway epithelium and changes with inflammation. We propose activation and/or luminal release of the inflammasome is a feature of allergic airway inflammation which may contribute to disease pathogenesis.
Description: Extent: 18p.
Rights: Copyright © 2012 Hai B. Tran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020127286
DOI: 10.1155/2012/819176
Appears in Collections:Surgery publications

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