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Type: Journal article
Title: Myeloid DAP12-associating lectin (MDL)-1 is a cell surface receptor involved in the activation of myeloid cells
Author: Baker, E.
Sutherland, G.
Phillips, J.
Lanier, L.
Citation: Proceedings of the National Academy of Sciences of USA, 1999; 96(17):9792-9796
Issue Date: 1999
ISSN: 0027-8424
Statement of
Baker, Elizabeth; Sutherland, Grant R.; Phillips, Joseph H.; Lanier, Lewis L.
Abstract: Crosslinking of immunoreceptor tyrosine-based activation motif (ITAM)-containing receptor complexes on a variety of cells leads to their activation through the sequential triggering of protein tyrosine kinases. Recently, DAP12 has been identified as an ITAM-bearing signaling molecule that is noncovalently associated with activating isoforms of MHC class I receptors on natural killer cells. In addition to natural killer cells, DAP12 is expressed in peripheral blood monocytes, macrophages, and dendritic cells, suggesting association with other receptors present in these cell types. In the present study, we report the molecular cloning of the myeloid DAP12-associating lectin-1 (MDL-1), a DAP12-associating membrane receptor expressed exclusively in monocytes and macrophages. MDL-1 is a type II transmembrane protein belonging to the C type lectin superfamily and contains a charged residue in the transmembrane region that enables it to pair with DAP12. Crosslinking of MDL-1/DAP12 complexes in J774 mouse macrophage cells resulted in calcium mobilization. These findings suggest that signaling via MDL-1/DAP12 complexes may constitute a significant activation pathway in myeloid cells.
Keywords: Killer Cells, Natural
Cell Line
Adaptor Proteins, Signal Transducing
Lectins, C-Type
Membrane Proteins
Receptors, Cell Surface
Receptors, Immunologic
Cloning, Molecular
Gene Expression Regulation
Amino Acid Sequence
Base Sequence
Surface Properties
Molecular Sequence Data
DOI: 10.1073/pnas.96.17.9792
Published version:
Appears in Collections:Aurora harvest 4
Paediatrics publications

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