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Type: Journal article
Title: Dasatinib targets chronic myeloid leukemia-CD34⁺ progenitors as effectively as it targets mature cells
Other Titles: Dasatinib targets chronic myeloid leukemia-CD34(+) progenitors as effectively as it targets mature cells
Author: Hiwase, D.
Saunders, V.
Nievergall, E.
Ross, D.
White, D.
Hughes, T.
Citation: Haematologica: the hematology journal, 2013; 98(6):896-900
Publisher: Ferrata Storti Foundation
Issue Date: 2013
ISSN: 0390-6078
Statement of
Devendra K. Hiwase, Verity A. Saunders, Eva Nievergall, Douglas D. Ross, Deborah L. White, and Timothy P. Hughes
Abstract: Dasatinib is effective in most chronic phase chronic myeloid leukemia patients both in first-line therapy and following imatinib failure. While imatinib uptake into CD34(+) cells is low compared to mononuclear cells, few data evaluate how well dasatinib targets primitive CML cells. This study compares intracellular concentration of dasatinib and Bcr-Abl kinase inhibition in CML-CD34(+) progenitors and mononuclear cells induced by dasatinib. The intracellular concentrations of dasatinib were similar between CML-CD34(+) and mononuclear cells (P=0.8). Similarly, there was no significant difference in the degree of dasatinib-mediated Bcr-Abl kinase inhibition. ABCB1 (MDR1) and ABCG2 inhibitors neither increased dasatinib intracellular concentration nor enhanced dasatinib-mediated Bcr-Abl kinase inhibition. In contrast to nilotinib, we show that dasatinib is not an ABCB1 inhibitor. Thus, dasatinib targets CML-CD34(+) progenitors as effectively as it targets mononuclear cells. ABCB1 and ABCG2 efflux pumps do not appear to influence the intracellular dasatinib concentration in CML-CD34(+) progenitors.
Keywords: Leukocytes, Mononuclear
Cells, Cultured
ATP-Binding Cassette Transporters
Neoplasm Proteins
Fusion Proteins, bcr-abl
Antineoplastic Agents
Antigens, CD34
Protein Kinase Inhibitors
Substrate Specificity
Neoplastic Stem Cells
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP Binding Cassette Transporter, Subfamily B
ATP Binding Cassette Transporter, Subfamily B, Member 1
Rights: ©2013 Ferrata Storti Foundation
DOI: 10.3324/haematol.2012.070268
Appears in Collections:Aurora harvest
Medicine publications

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