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Type: Journal article
Title: Complex regulation by Apetala2 domain-containing transcription factors revealed through analysis of the stress-responsive TdCor410b promoter from durum wheat
Author: Eini Gandomani, O.
Yang, N.
Pyvovarenko, T.
Pillman, K.
Bazanova, N.
Tikhomirov, N.
Eliby, S.
Shirley, N.
Sivasankar, S.
Tingey, S.
Langridge, P.
Hrmova, M.
Lopato, S.
Citation: PLoS One, 2013; 8(3):1-19
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Statement of
Omid Eini, Nannan Yang, Tatiana Pyvovarenko, Katherine Pillman, Natalia Bazanova, Natalia Tikhomirov, Serik Eliby, Neil Shirley, Shoba Sivasankar, Scott Tingey, Peter Langridge, Maria Hrmova, Sergiy Lopato
Abstract: Expression of the wheat dehydrin gene Cor410b is induced several fold above its non-stressed levels upon exposure to stresses such as cold, drought and wounding. Deletion analysis of the TdCor410b promoter revealed a single functional C-repeat (CRT) element. Seven transcription factors (TFs) were shown to bind to this CRT element using yeast one-hybrid screens of wheat and barley cDNA libraries, of which only one belonged to the DREB class of TFs. The remaining six encoded ethylene response factors (ERFs) belong to three separate subfamilies. Analysis of binding selectivity of these TFs indicated that all seven could bind to the CRT element (GCCGAC), and that three of the six ERFs could bind both to the CRT element and the ethylene-responsive GCC-box (GCCGCC). The TaERF4 subfamily members specifically bound the CRT element, and did not bind either the GCC-box or DRE element (ACCGAC). Molecular modeling and site-directed mutagenesis identified a single residue Pro42 in the Apetala2 (AP2) domain of TaERF4-like proteins that is conserved in monocotyledonous plants and is responsible for the recognition selectivity of this subfamily. We suggest that both DREB and ERF proteins regulate expression of the Cor410b gene through a single, critical CRT element. Members of the TaERF4 subfamily are specific, positive regulators of Cor410b gene expression.
Keywords: Plants, Genetically Modified
Plant Proteins
Transcription Factors
DNA, Plant
Gene Expression Regulation, Plant
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Genes, Plant
Models, Molecular
Molecular Sequence Data
Promoter Regions, Genetic
Stress, Physiological
Rights: Copyright: © 2013 Eini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0058713
Grant ID: ARC
Appears in Collections:Agriculture, Food and Wine publications
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