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Type: Journal article
Title: Prostaglandin ethanolamides attenuate damage in a human explant colitis model
Author: Nicotra, L.
Vu, M.
Harvey, B.
Smid, S.
Citation: Prostaglandins & Other Lipid Mediators, 2013; 100(1):22-29
Publisher: Elsevier Inc.
Issue Date: 2013
ISSN: 1098-8823
Statement of
Lauren L. Nicotra, Megan Vu, Benjamin S. Harvey and Scott D. Smid
Abstract: Endocannabinoids are protective in animal colitis models. As endocannabinoids also form novel prostaglandin ethanolamides (prostamides) via COX-2, we investigated the effects of prostamides and other COX-2 mediators on tissue damage in an ex vivo human mucosal explant colitis model. Healthy human colonic mucosae were incubated with pro-inflammatory cytokines TNF-α and IL-1β to elicit colitis-like tissue damage. The PGF-ethanolamide analogue, bimatoprost decreased colitis scores which were reversed by a prostamide-specific antagonist AGN 211334, but not the FP receptor antagonist AL-8810. PGF-ethanolamide and PGE-ethanolamide also reduced cytokine-evoked epithelial damage. Anandamide was protective in the explant colitis model; however COX-2 inhibition did not alter its effects, associated with a lack of COX-2 induction in explant mucosal tissue. These findings support an anti-inflammatory role for prostamides and endocannabinoids in the human colon.
Keywords: Colon, Sigmoid; Humans; Colitis; Amides; Sulfonamides; Oxazoles; Arachidonic Acids; Dinoprostone; Dinoprost; Cloprostenol; Tumor Necrosis Factor-alpha; Receptors, Prostaglandin; Cyclooxygenase Inhibitors; Endocannabinoids; Tissue Culture Techniques; Immunohistochemistry; Adult; Middle Aged; Female; Male; Cyclooxygenase 2; Polyunsaturated Alkamides; Interleukin-1beta; Young Adult; Bimatoprost
Rights: © 2013 Elsevier Inc. All rights reserved.
RMID: 0020126615
DOI: 10.1016/j.prostaglandins.2013.01.003
Appears in Collections:Pharmacology publications

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