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dc.contributor.authorHudson Keenihan, S.-
dc.contributor.authorRobertson, S.-
dc.identifier.citationBiology of Reproduction, 2004; 70(6):1562-1572-
dc.description© 2004 by the Society for the Study of Reproduction, Inc.-
dc.description.abstractThe dendritic cells and related antigen-presenting cells (APCs) that activate lymphocytes for acquired immunity in the female reproductive tract are not well characterized. The aim of the present study was to examine heterogeneity among uterine APCs in mice and, specifically, to determine whether phenotypically and functionally distinct subpopulations of dendritic cells and macrophages can be identified. Using immunohistochemistry, abundant cells expressing APC-restricted molecules major histocompatibility complex (MHC) class II, F4/80, class A scavenger receptor, macrosialin, and sialoadhesin were evident in estrous mice. Cells expressing the costimulatory molecule B7-2 were rarely observed. Flow cytometric analysis revealed three subpopulations of uterine APCs. Undifferentiated macrophages were F4/80-positive (+), MHC class II-negative (–) cells, of which 70–80% expressed CD11b, but few expressed class A scavenger receptor, macrosialin, or sialoadhesin. Mature macrophages were F4/80+/MHC class II+ cells, of which approximately 50% expressed CD11b, class A scavenger receptor, macrosialin, and sialoadhesin. Uterine dendritic cells were F4/ 80–/MHC class II+ cells, with stimulatory immunoaccessory function relative to uterine macrophages and heterogeneous expression of dendritic markers 33D1, DEC205, CD11c, and CD1. Experiments in ovariectomized mice showed that undifferentiated macrophages were steroid hormone dependent but that mature macrophages and dendritic cells persisted after depletion of ovarian steroid hormones, although with altered phenotypes. In summary, our findings identify three discrete populations of APCs inhabiting the murine uterus and suggest that both mature macrophages and dendritic cells differentiate from undifferentiated macrophage precursor cells. Plasticity in the ontogenetic and functional relationships between uterine dendritic cells and macrophages likely is critical in regulating immune responses conducive to reproductive success.-
dc.description.statementofresponsibilitySarah N. Hudson Keenihan, and Sarah A. Robertson-
dc.publisherSoc Study Reproduction-
dc.subjectDendritic Cells-
dc.subjectMice, Inbred BALB C-
dc.subjectMice, Inbred C57BL-
dc.subjectMembrane Glycoproteins-
dc.subjectReceptors, Immunologic-
dc.subjectAntigens, Differentiation-
dc.subjectAntigens, CD-
dc.subjectAntigens, CD1-
dc.subjectAntigens, Differentiation, Myelomonocytic-
dc.subjectHistocompatibility Antigens Class II-
dc.subjectCell Differentiation-
dc.subjectReceptors, Scavenger-
dc.subjectScavenger Receptors, Class A-
dc.subjectSialic Acid Binding Ig-like Lectin 1-
dc.subjectCD11b Antigen-
dc.titleDiversity in phenotype and steroid hormone dependence in dendritic cells and macrophages in the mouse uterus-
dc.typeJournal article-
dc.identifier.orcidRobertson, S. [0000-0002-9967-0084]-
Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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