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https://hdl.handle.net/2440/8055
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dc.contributor.author | Robertson, S. | - |
dc.contributor.author | Mayrhofer, G. | - |
dc.contributor.author | Seamark, R. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Biology of Reproduction, 1996; 54(1):183-196 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.issn | 1529-7268 | - |
dc.identifier.uri | http://hdl.handle.net/2440/8055 | - |
dc.description.abstract | Uterine epithelial cells have been shown by in vitro studies to be a potent source of the inflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF), and the luminal and glandular epithelium has been confirmed as the predominant site of GM-CSF expression in the intact endometrium by in situ hybridization. To examine the role of ovarian steroid hormones in GM-CSF synthesis, GM-CSF bioactivity has been measured in the supernatants of short-term primary cultures of endometrial cells prepared from mice in which steroid levels were perturbed by ovariectomy and steroid replacement or by steroid antagonists. GM-CSF production was found to fluctuate in cells harvested at different times during the estrous cycle, peaking at estrus. Endometrial cells derived from ovariectomized mice produced 25-fold less GM-CSF than did cells from estrous mice, and production was increased if ovariectomized mice were pretreated with estrogen, but not progesterone, 3 h or more before harvest. This estrogen-induced increase was inhibited by coadministration of progesterone or by induction of a decidual response and was blocked by the estrogen antagonist ZK 119,010. By contrast, pretreatment of mice with the anti-progestin RU486 significantly elevated GM-CSF output in cells from ovariectomized mice given estrogen and progesterone in combination and antagonized the inhibition of GM-CSF release seen in cells harvested from mice treated with hCG. These studies demonstrate that GM-CSF synthesis and/or release by uterine epithelial cells is stimulated by estrogen, with progesterone having a moderate inhibitory effect. Analysis of GM-CSF mRNA expression in uterine epithelial cell cultures and in intact uteri from steroid hormone-treated ovariectomized mice by quantitative reverse transcription-polymerase chain reaction indicated that the effects of estrogen and progesterone on GM-CSF release are mediated at least in part at the transcriptional level. These findings implicate GM-CSF as a local mediator of steroid-driven remodeling events in the cycling and preimplantation endometrium, possibly acting through the recruitment and behavioral regulation of granulocytes and macrophages. | - |
dc.language.iso | en | - |
dc.publisher | SOC STUDY REPRODUCTION | - |
dc.source.uri | http://dx.doi.org/10.1095/biolreprod54.1.183 | - |
dc.subject | Ovary | - |
dc.subject | Uterus | - |
dc.subject | Decidua | - |
dc.subject | Epithelium | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Mice, Inbred C57BL | - |
dc.subject | Mice | - |
dc.subject | Estradiol | - |
dc.subject | Progesterone | - |
dc.subject | Chorionic Gonadotropin | - |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | RNA, Messenger | - |
dc.subject | Ovariectomy | - |
dc.subject | In Situ Hybridization | - |
dc.subject | Estrus | - |
dc.subject | Kinetics | - |
dc.subject | Female | - |
dc.title | Ovarian steroid hormones regulate granulocyte-macrophage colony-stimulating factor synthesis by uterine epithelial cells in the mouse | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1095/biolreprod54.1.183 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robertson, S. [0000-0002-9967-0084] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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