Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/8055
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dc.contributor.authorRobertson, S.-
dc.contributor.authorMayrhofer, G.-
dc.contributor.authorSeamark, R.-
dc.date.issued1996-
dc.identifier.citationBiology of Reproduction, 1996; 54(1):183-196-
dc.identifier.issn0006-3363-
dc.identifier.issn1529-7268-
dc.identifier.urihttp://hdl.handle.net/2440/8055-
dc.description.abstractUterine epithelial cells have been shown by in vitro studies to be a potent source of the inflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF), and the luminal and glandular epithelium has been confirmed as the predominant site of GM-CSF expression in the intact endometrium by in situ hybridization. To examine the role of ovarian steroid hormones in GM-CSF synthesis, GM-CSF bioactivity has been measured in the supernatants of short-term primary cultures of endometrial cells prepared from mice in which steroid levels were perturbed by ovariectomy and steroid replacement or by steroid antagonists. GM-CSF production was found to fluctuate in cells harvested at different times during the estrous cycle, peaking at estrus. Endometrial cells derived from ovariectomized mice produced 25-fold less GM-CSF than did cells from estrous mice, and production was increased if ovariectomized mice were pretreated with estrogen, but not progesterone, 3 h or more before harvest. This estrogen-induced increase was inhibited by coadministration of progesterone or by induction of a decidual response and was blocked by the estrogen antagonist ZK 119,010. By contrast, pretreatment of mice with the anti-progestin RU486 significantly elevated GM-CSF output in cells from ovariectomized mice given estrogen and progesterone in combination and antagonized the inhibition of GM-CSF release seen in cells harvested from mice treated with hCG. These studies demonstrate that GM-CSF synthesis and/or release by uterine epithelial cells is stimulated by estrogen, with progesterone having a moderate inhibitory effect. Analysis of GM-CSF mRNA expression in uterine epithelial cell cultures and in intact uteri from steroid hormone-treated ovariectomized mice by quantitative reverse transcription-polymerase chain reaction indicated that the effects of estrogen and progesterone on GM-CSF release are mediated at least in part at the transcriptional level. These findings implicate GM-CSF as a local mediator of steroid-driven remodeling events in the cycling and preimplantation endometrium, possibly acting through the recruitment and behavioral regulation of granulocytes and macrophages.-
dc.language.isoen-
dc.publisherSOC STUDY REPRODUCTION-
dc.subjectOvary-
dc.subjectUterus-
dc.subjectDecidua-
dc.subjectEpithelium-
dc.subjectAnimals-
dc.subjectMice, Inbred BALB C-
dc.subjectMice, Inbred C57BL-
dc.subjectMice-
dc.subjectEstradiol-
dc.subjectProgesterone-
dc.subjectChorionic Gonadotropin-
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subjectRNA, Messenger-
dc.subjectOvariectomy-
dc.subjectIn Situ Hybridization-
dc.subjectEstrus-
dc.subjectKinetics-
dc.subjectFemale-
dc.titleOvarian steroid hormones regulate granulocyte-macrophage colony-stimulating factor synthesis by uterine epithelial cells in the mouse-
dc.typeJournal article-
dc.identifier.doi10.1095/biolreprod54.1.183-
pubs.publication-statusPublished-
dc.identifier.orcidRobertson, S. [0000-0002-9967-0084]-
Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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