Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/80757
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Type: Journal article
Title: Interplay between manganese and iron in pneumococcal pathogenesis: Role of the orphan response regulator ritR
Author: Ong, C.
Potter, A.
Trappetti, C.
Walker, M.
Jennings, M.
Paton, J.
McEwan, A.
Citation: Infection and Immunity, 2013; 81(2):421-429
Publisher: Amer Soc Microbiology
Issue Date: 2013
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Cheryl-Lynn Y. Ong, Adam J. Potter, Claudia Trappetti, Mark J. Walker, Michael P. Jennings, James C. Paton, Alastair G. McEwan
Abstract: Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that is carried asymptomatically in the nasopharynx by up to 70% of the human population. Translocation of the bacteria into internal sites can cause a range of diseases, such as pneumonia, otitis media, meningitis, and bacteremia. This transition from nasopharynx to growth at systemic sites means that the pneumococcus needs to adjust to a variety of environmental conditions, including transition metal ion availability. Although it is an important nutrient, iron potentiates oxidative stress, and it is established that in S. pneumoniae, expression of iron transport systems and proteins that protect against oxidative stress are regulated by an orphan response regulator, RitR. In this study, we investigated the effect of iron and manganese ion availability on the growth of a ritR mutant. Deletion of ritR led to impaired growth of bacteria in high-iron medium, but this phenotype could be suppressed with the addition of manganese. Measurement of metal ion accumulation indicated that manganese prevents iron accumulation. Furthermore, the addition of manganese also led to a reduction in the amount of hydrogen peroxide produced by bacterial cells. Studies of virulence in a murine model of infection indicated that RitR was not essential for pneumococcal survival and suggested that derepression of iron uptake systems may enhance the survival of pneumococci in some niches.
Keywords: Animals; Mice; Streptococcus pneumoniae; Pneumococcal Infections; Hydrogen Peroxide; Iron; Manganese; Bacterial Proteins; Cation Transport Proteins; Virulence; Gene Expression Regulation, Bacterial; Ion Transport; Oxidative Stress; Mutation; Female
Rights: Copyright © 2013, American Society for Microbiology. All Rights Reserved.
RMID: 0020124788
DOI: 10.1128/IAI.00805-12
Appears in Collections:Molecular and Biomedical Science publications

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