Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Interplay between manganese and iron in pneumococcal pathogenesis: Role of the orphan response regulator ritR
Author: Ong, C.
Potter, A.
Trappetti, C.
Walker, M.
Jennings, M.
Paton, J.
McEwan, A.
Citation: Infection and Immunity, 2013; 81(2):421-429
Publisher: Amer Soc Microbiology
Issue Date: 2013
ISSN: 0019-9567
Statement of
Cheryl-Lynn Y. Ong, Adam J. Potter, Claudia Trappetti, Mark J. Walker, Michael P. Jennings, James C. Paton, Alastair G. McEwan
Abstract: Streptococcus pneumoniae (the pneumococcus) is a major human pathogen that is carried asymptomatically in the nasopharynx by up to 70% of the human population. Translocation of the bacteria into internal sites can cause a range of diseases, such as pneumonia, otitis media, meningitis, and bacteremia. This transition from nasopharynx to growth at systemic sites means that the pneumococcus needs to adjust to a variety of environmental conditions, including transition metal ion availability. Although it is an important nutrient, iron potentiates oxidative stress, and it is established that in S. pneumoniae, expression of iron transport systems and proteins that protect against oxidative stress are regulated by an orphan response regulator, RitR. In this study, we investigated the effect of iron and manganese ion availability on the growth of a ritR mutant. Deletion of ritR led to impaired growth of bacteria in high-iron medium, but this phenotype could be suppressed with the addition of manganese. Measurement of metal ion accumulation indicated that manganese prevents iron accumulation. Furthermore, the addition of manganese also led to a reduction in the amount of hydrogen peroxide produced by bacterial cells. Studies of virulence in a murine model of infection indicated that RitR was not essential for pneumococcal survival and suggested that derepression of iron uptake systems may enhance the survival of pneumococci in some niches.
Keywords: Animals
Streptococcus pneumoniae
Pneumococcal Infections
Hydrogen Peroxide
Bacterial Proteins
Cation Transport Proteins
Gene Expression Regulation, Bacterial
Ion Transport
Oxidative Stress
Rights: Copyright © 2013, American Society for Microbiology. All Rights Reserved.
DOI: 10.1128/IAI.00805-12
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.